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Literature summary extracted from

  • Maley, G.F.
    Deoxycytidylate deaminase from T2-infected Escherichia coli (1978), Methods Enzymol., 51, 412-418.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 5-hydroxymethyl-dCTP
-
 Escherichia phage T2
3.5.4.12 dCTP positive allosteric effector Escherichia phage T2

General Stability

EC Number General Stability Organism
3.5.4.12 2-mercaptoethanol stabilizes Escherichia phage T2
3.5.4.12 dCTP, or to a lesser extent dTTP, stabilize against heat inactivation, protein denaturants and proteolytic enzymes Escherichia phage T2
3.5.4.12 thiols stabilize Escherichia phage T2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 dTTP allosteric inhibitor Escherichia phage T2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.12 additional information
-
 additional information kinetic properties and parameters Escherichia phage T2
3.5.4.12 0.1
-
 dCMP pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km Escherichia phage T2
3.5.4.12 0.1
-
 5-methyl-dCMP pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km Escherichia phage T2
3.5.4.12 10
-
 CMP pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km Escherichia phage T2

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.12 Ca2+ reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+ Escherichia phage T2
3.5.4.12 divalent cations reaction completely dependent on divalent cations Escherichia phage T2
3.5.4.12 Mg2+ reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+ Escherichia phage T2
3.5.4.12 Mn2+ reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+ Escherichia phage T2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.12 124000
-
 PAGE and sedimentation equilibrium Escherichia phage T2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Escherichia phage T2 true substrate probably dCMP-Mg complex dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O Escherichia phage T2 pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP dUMP + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Escherichia phage T2
-
 T2-phage infected Escherichia coli
-
3.5.4.12 no activity in Escherichia coli
-
 enzyme not detectable in Escherichia coli until after infection with T-even bacteriophage
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12 T2-phage infected Escherichia coli Escherichia phage T2

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Escherichia phage T2
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.12 430
-
-
 Escherichia phage T2

Storage Stability

EC Number Storage Stability Organism
3.5.4.12 0-4°C, neutral pH, 0.1 M 2-mercaptoethanol, stable for several months Escherichia phage T2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 5-bromo-dCMP + H2O
-
 Escherichia phage T2 5-bromo-dUMP + NH3
-
 ?
3.5.4.12 5-chloro-dCMP + H2O
-
 Escherichia phage T2 5-chloro-dUMP + NH3
-
 ?
3.5.4.12 5-fluoro-dCMP + H2O
-
 Escherichia phage T2 5-fluoro-dUMP + NH3
-
 ?
3.5.4.12 5-hydroxymethyl-dCMP + H2O very poor substrate Escherichia phage T2 5-hydroxymethyl-dUMP + NH3
-
 ?
3.5.4.12 5-iodo-dCMP + H2O very poor substrate Escherichia phage T2 5-iodo-dUMP + NH3
-
 ?
3.5.4.12 5-methyl-dCMP + H2O
-
 Escherichia phage T2 5-methyl-dUMP + NH3
-
 ?
3.5.4.12 CMP + H2O
-
 Escherichia phage T2 UMP + NH3
-
 ?
3.5.4.12 dCMP + H2O true substrate probably dCMP-Mg complex Escherichia phage T2 dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP Escherichia phage T2 dUMP + NH3
-
 ?
3.5.4.12 additional information substrate specificity Escherichia phage T2 ?
-
 ?
3.5.4.12 additional information catalyzes deamination of halogenated derivatives of dCMP, but appears to be restricted in its ability to deaminate substrates with a large group in the 5-position Escherichia phage T2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.12 hexamer 6 identical subunits Escherichia phage T2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.12 additional information
-
 dCTP, and to a lesser extent dTTP, stabilizes against heat inactivation Escherichia phage T2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.12 5.3
-
 in the absence of dCTP Escherichia phage T2
3.5.4.12 8
-
 assay at Escherichia phage T2
3.5.4.12 8.3
-
 in the presence of dCTP or 5-hydroxymethyl-dCTP Escherichia phage T2