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Literature summary extracted from
- Deoxycytidylate deaminase. Purification and some properties of the enzyme isolated from human spleen (1981), J. Biol. Chem., 256, 6335-6340.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.12 | 1-beta-D-arabinofuranosylcytosine 5'-triphosphate | low activation, allosteric activator | Homo sapiens |  |
| 3.5.4.12 | CDP | low activation, allosteric activator | Homo sapiens | |
| 3.5.4.12 | CTP | low activation, allosteric activator | Homo sapiens | |
| 3.5.4.12 | dAMP | 0.025 mM: low activation | Homo sapiens | |
| 3.5.4.12 | dCDP | low activation, allosteric activator | Homo sapiens | |
| 3.5.4.12 | dCTP | allosteric activator | Homo sapiens | |
| 3.5.4.12 | dCTP | less than 0.001 mM: very high activation | Homo sapiens | |
| 3.5.4.12 | dCTP | activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP | Homo sapiens | |
| 3.5.4.12 | TTP | activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition | Homo sapiens | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.5.4.12 | dCTP and MgCl2 stabilize | Homo sapiens |
| 3.5.4.12 | dithiothreitol, 2 mM, stabilizes | Homo sapiens |
| 3.5.4.12 | glycerol, 15%, stabilizes | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.12 | ADP | slight inhibition | Homo sapiens | |
| 3.5.4.12 | AMP | slight inhibition, competitive inhibitor, mechanism of inhibition | Homo sapiens | |
| 3.5.4.12 | Co2+ | 1 mM: 75% inhibition | Homo sapiens | |
| 3.5.4.12 | Cu2+ | 1 mM: 98% inhibition | Homo sapiens | |
| 3.5.4.12 | dGDP | slight inhibition | Homo sapiens | |
| 3.5.4.12 | dGMP | effective competitive inhibitor, mechanism of inhibition | Homo sapiens | |
| 3.5.4.12 | dUMP | poor competitive inhibitor, mechanism of inhibition | Homo sapiens | |
| 3.5.4.12 | dUTP | slight inhibition | Homo sapiens | |
| 3.5.4.12 | EDTA | no inhibition by EDTA | Homo sapiens | |
| 3.5.4.12 | GMP | slight inhibition | Homo sapiens | |
| 3.5.4.12 | additional information | no inhibition by EDTA | Homo sapiens | |
| 3.5.4.12 | TDP | slight inhibition, competitive inhibitor, mechanism of inhibition | Homo sapiens | |
| 3.5.4.12 | TMP | poor competitive inhibitor, mechanism of inhibition | Homo sapiens | |
| 3.5.4.12 | TTP | activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP | Homo sapiens | |
| 3.5.4.12 | UTP | slight inhibition | Homo sapiens | |
| 3.5.4.12 | Zn2+ | 1 mM: 94% inhibition | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.12 | additional information | - |
additional information | kinetic behavior and data | Homo sapiens |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.12 | Ca2+ | activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP | Homo sapiens | |
| 3.5.4.12 | Mg2+ | activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP | Homo sapiens | |
| 3.5.4.12 | Mn2+ | activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP | Homo sapiens | |
| 3.5.4.12 | additional information | metal ions not required | Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.12 | 53000 | - |
2 * 53000, SDS-PAGE | Homo sapiens |
| 3.5.4.12 | 109500 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.12 | dCMP + H2O | Homo sapiens | important role in regulation of intracellular TTP levels | dUMP + NH3 | - |
ir | |
| 3.5.4.12 | dCMP + H2O | Homo sapiens | enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP | dUMP + NH3 | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.12 | Homo sapiens | - |
human | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.12 | - |
Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.4.12 | dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Homo sapiens | |
| 3.5.4.12 | dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.4.12 | spleen | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.12 | 10 | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.4.12 | -50°C, 0.05 mM dCTP, 1 mM MgCl2 or 2 mM dithiothreitol, 18 h, 40% activity lost | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.12 | ara-CMP + H2O | arabinosyl-CMP | Homo sapiens | ara-UMP + NH3 | - |
? | |
| 3.5.4.12 | ara-CMP + H2O | 1-beta-D-arabinofuranosyl-CMP | Homo sapiens | ara-UMP + NH3 | - |
? | |
| 3.5.4.12 | ara-CMP + H2O | effective substrate in presence of dCTP | Homo sapiens | ara-UMP + NH3 | - |
? | |
| 3.5.4.12 | CMP + H2O | poor substrate | Homo sapiens | UMP + NH3 | - |
? | |
| 3.5.4.12 | dCMP + H2O | high degree of specificity of dCMP | Homo sapiens | dUMP + NH3 | - |
ir | |
| 3.5.4.12 | dCMP + H2O | important role in regulation of intracellular TTP levels | Homo sapiens | dUMP + NH3 | - |
ir | |
| 3.5.4.12 | dCMP + H2O | enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP | Homo sapiens | dUMP + NH3 | - |
ir | |
| 3.5.4.12 | additional information | substrate specificity | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.12 | dimer | 2 * 53000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.12 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.12 | additional information | - |
pI: 5.10 | Homo sapiens |
| 3.5.4.12 | 6.5 | 7.3 | broad pH optimum | Homo sapiens |
| 3.5.4.12 | 7.2 | - |
assay at | Homo sapiens |
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