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Literature summary extracted from

  • Yoshimoto, T.; Oka, I.; Tsuru, D.
    Creatine amidinohydrolase of Pseudomonas putida: crystallization and some properties (1976), Arch. Biochem. Biophys., 177, 508-515.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.3.3
-
 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.3 CaCl2 1 mM, 32% inhibition Pseudomonas putida
3.5.3.3 CoCl2 1 mM, 71% inhibition Pseudomonas putida
3.5.3.3 Cu2+ 1 mM CuSO4, complete inhibition Pseudomonas putida
3.5.3.3 DFP 1 mM, 30% inhibition Pseudomonas putida
3.5.3.3 EDTA 2 mM, 10% inhibition Pseudomonas putida
3.5.3.3 FeCl3 1 mM, 71% inhibition Pseudomonas putida
3.5.3.3 Hg2+ 1 mM HgCl2, complete inhibition Pseudomonas putida
3.5.3.3 MgSO4 1 mM, 31% inhibition Pseudomonas putida
3.5.3.3 N-bromosuccinimide 1 mM, 30% inhibition Pseudomonas putida
3.5.3.3 Pb-Acetate 1 mM, 15% inhibition Pseudomonas putida
3.5.3.3 PCMB 0.5 mM, complete inhibition Pseudomonas putida
3.5.3.3 Zn2+ 1 mM ZnCl2, complete inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.3 1.33
-
 Creatine
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.3 47000
-
 2 * 47000, SDS-PAGE Pseudomonas putida
3.5.3.3 94000
-
 meniscus depletion method Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.3 Pseudomonas putida
-
 var. naraensis C-83
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.3
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.3 0.128
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.3 creatine + H2O
-
 Pseudomonas putida sarcosine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.3 dimer 2 * 47000, SDS-PAGE Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.3 0.246
-
 Creatine
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.3 8
-
-
 Pseudomonas putida