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Literature summary extracted from

  • Ogawa, T.; Kimoto, M.; Sasaoka, K.
    Purification and properties of a new enzyme, NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney (1989), J. Biol. Chem., 264, 10205-10209.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.18 Cd2+
-
 Rattus norvegicus
3.5.3.18 Cu2+
-
 Rattus norvegicus
3.5.3.18 HgCl2
-
 Rattus norvegicus
3.5.3.18 L-citrulline competitive Rattus norvegicus
3.5.3.18 NEM
-
 Rattus norvegicus
3.5.3.18 PCMB
-
 Rattus norvegicus
3.5.3.18 Zn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.18 0.18
-
 NG,NG-dimethyl-L-arginine
-
 Rattus norvegicus
3.5.3.18 0.36
-
 NG-monomethyl-L-arginine
-
 Rattus norvegicus
3.5.3.18 9.24
-
 NG,NG-dimethyl-L-arginine
-
 Rattus norvegicus
3.5.3.18 33
-
 NG,NG-Dimethyl-L-homoarginine
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.18 32000
-
 gel filtration Rattus norvegicus
3.5.3.18 34000
-
 1 * 34000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.18 NG,NG-dimethyl-L-arginine + H2O Rattus norvegicus enzyme plays a role in metabolism of NG,NG-dimethyl-L-arginine dimethylamine + L-citrulline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.18 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.18
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.18 brain
-
 Rattus norvegicus
-
3.5.3.18 kidney
-
 Rattus norvegicus
-
3.5.3.18 liver
-
 Rattus norvegicus
-
3.5.3.18 lung
-
 Rattus norvegicus
-
3.5.3.18 pancreas
-
 Rattus norvegicus
-
3.5.3.18 skeletal muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.18 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.5.3.18 -30°C, 0.1 M sodium phosphate buffer, pH 7.0, 10% glycerol, 1 mM 2-mercaptoethanol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.18 NG,NG-dimethyl-L-arginine + H2O
-
 Rattus norvegicus dimethylamine + L-citrulline
-
 ir
3.5.3.18 NG,NG-dimethyl-L-arginine + H2O enzyme plays a role in metabolism of NG,NG-dimethyl-L-arginine Rattus norvegicus dimethylamine + L-citrulline
-
 ?
3.5.3.18 NG,NG-dimethyl-L-homoarginine + H2O at 2.8% of the activity with NG,NG-dimethyl-L-arginine Rattus norvegicus ?
-
 ?
3.5.3.18 NG-monomethyl-L-arginine + H2O at 61% of the activity with NG,NG-dimethyl-L-arginine Rattus norvegicus citrulline + methylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.18 monomer 1 * 34000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.18 55
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.18 additional information
-
-
 Rattus norvegicus
3.5.3.18 45
-
 stable Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.18 0.009
-
 NG,NG-Dimethyl-L-homoarginine
-
 Rattus norvegicus
3.5.3.18 0.094
-
 NG-monomethyl-L-arginine
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.18 6.5
-
 hydrolysis of NG,NG-dimethyl-L-arginine Rattus norvegicus
3.5.3.18 6.7
-
 hydrolysis of NG-monomethyl-L-arginine Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.18 5 8.5 stable between Rattus norvegicus