EC Number | General Stability | Organism |
---|---|---|
3.5.3.18 | Zn2+ has a structure-stabilizing role | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.18 | Zn2+ | besides the tightly bound zinc, additional Zn2+ inhibit competitively | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.18 | 1.31 | - |
NG,NG-dimethyl-L-arginine | - |
Bos taurus | |
3.5.3.18 | 1.6 | - |
NG-monomethyl-L-arginine | - |
Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.18 | Zn2+ | contains one tightly bound zinc ion | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.18 | NG,NG-dimethyl-L-arginine + H2O | Bos taurus | enzyme is involved in regulation of the levels of the natural occuring free arginine derivatives L-Nomega,Nomega-dimethylarginine and L-Nomega-methylarginine, which are reversible inhibitors of nitric oxide synthase | dimethylamine + L-citrulline | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.3.18 | Bos taurus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.3.18 | brain | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.18 | NG,NG-dimethyl-L-arginine + H2O | - |
Bos taurus | dimethylamine + L-citrulline | - |
? | |
3.5.3.18 | NG,NG-dimethyl-L-arginine + H2O | enzyme is involved in regulation of the levels of the natural occuring free arginine derivatives L-Nomega,Nomega-dimethylarginine and L-Nomega-methylarginine, which are reversible inhibitors of nitric oxide synthase | Bos taurus | dimethylamine + L-citrulline | - |
? | |
3.5.3.18 | NG-monomethyl-L-arginine + H2O | - |
Bos taurus | citrulline + methylamine | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.18 | 50 | - |
2 h, holo-enzyme retains ca. 56% of the original activity, no residual activity of the apoenzyme. Thermal denaturation of both protein forms is irreversible | Bos taurus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.18 | 0.27 | - |
NG,NG-dimethyl-L-arginine | - |
Bos taurus | |
3.5.3.18 | 0.34 | - |
NG-monomethyl-L-arginine | - |
Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.18 | 6.2 | - |
hydrolysis of NG,NG-dimethyl-L-arginine | Bos taurus |