Literature summary extracted from
Milne, K.G.; Field, R.A.; Masterson, W.J.; Cooaz, S.; Brimacombe, J.S.; Ferguson, M.A.J.
Partial purification and characterization of the N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol anchor biosynthesis in African trypanosomes (1994), J. Biol. Chem., 269, 16403-16408.
General Stability
EC Number |
General Stability |
Organism |
---|
3.5.1.89 |
10-50% v/v glycerol increases stability of solubilized enzyme |
Trypanosoma sp. |
3.5.1.89 |
Solubilized enzyme loses activity upon freeze-thawing |
Trypanosoma sp. |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.5.1.89 |
0.00015 |
- |
N-acetyl-D-glucosaminylphosphatidylinositol |
- |
Trypanosoma sp. |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.5.1.89 |
membrane |
- |
Trypanosoma sp. |
16020 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.1.89 |
Trypanosoma sp. |
- |
variant MITat 1.4 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.5.1.89 |
variant MITat 1.4, partial |
Trypanosoma sp. |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.5.1.89 |
bloodstream form |
of Trypanosoma |
Trypanosoma sp. |
- |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
3.5.1.89 |
Highly purified preparation is extremely labile, even when stored in 50% glycerol |
Trypanosoma sp. |
3.5.1.89 |
Solubilized enzyme loses activity upon prolonged storage at 4°C |
Trypanosoma sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.1.89 |
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O |
analogues lacking the glycerophosphate component are not recognized |
Trypanosoma sp. |
D-Glucosaminylphosphatidylinositol + acetate |
- |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.5.1.89 |
7.4 |
- |
solubilized enzyme |
Trypanosoma sp. |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.5.1.89 |
6.4 |
8 |
90% of activity maximum at pH 6.4 and 8.0, solubilized enzyme |
Trypanosoma sp. |