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Literature summary extracted from

  • Ogawa, J.; Miyake, H.; Shimizu, S.
    Purification and characterization of N-carbamoyl-L-amino acid amidohydrolase with broad substrate specificity from Alcaligenes xylosoxidans (1995), Appl. Microbiol. Biotechnol., 43, 1039-1043.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.87 5,5'-dithiobis(2-nitrobenzoic acid) 2 mM, 100% inhibition Achromobacter xylosoxidans
3.5.1.87 8-hydroxyquinoline 2 mM, 65% inhibition Achromobacter xylosoxidans
3.5.1.87 alpha,alpha'-dipyridyl 2.5 mM, 26% inhibition Achromobacter xylosoxidans
3.5.1.87 Cd2+ 2 mM, 86% inhibition Achromobacter xylosoxidans
3.5.1.87 Cu2+ 2 mM, 85% inhibition Achromobacter xylosoxidans
3.5.1.87 diisopropyl fluorophosphate 5 mM, 18% inhibition Achromobacter xylosoxidans
3.5.1.87 EDTA 10 mM, 22% inhibition Achromobacter xylosoxidans
3.5.1.87 Hg2+ 2 mM, 100% inhibition Achromobacter xylosoxidans
3.5.1.87 iodoacetate 2 mM, 43% inhibitiion Achromobacter xylosoxidans
3.5.1.87 additional information not inhibited by 5 mM NaCN, NaF, NaN3, NH2OH, semicarbazide, (NH4)2SO4, NaCO3, N-carbamoyl-D-valine, N-carbamoyl-D-alanine or L-alanine Achromobacter xylosoxidans
3.5.1.87 N-ethylmaleimide 2 mM, 100% inhibitiion Achromobacter xylosoxidans
3.5.1.87 NaAsO2 5 mM, 20% inhibition Achromobacter xylosoxidans
3.5.1.87 o-phenanthroline 10 mM, 47% inhibition Achromobacter xylosoxidans
3.5.1.87 p-chloromercuribenzoic acid 2 mM, 100% inhibitiion Achromobacter xylosoxidans
3.5.1.87 phenylhydrazine 5 mM, 48% inhibition Achromobacter xylosoxidans
3.5.1.87 phenylmethylsulfonyl fluoride 5 mM, 31% inhibition Achromobacter xylosoxidans
3.5.1.87 sodium propionate 10 mM, 20% inhibition, competitive inhibition of N-carbamoyl-L-alanine hydrolysis Achromobacter xylosoxidans
3.5.1.87 Zn2+ 2 mM, 85% inhibition Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.87 0.31
-
 N-carbamoyl-L-isoleucine
-
 Achromobacter xylosoxidans
3.5.1.87 0.4
-
 N-carbamoyl-L-valine
-
 Achromobacter xylosoxidans
3.5.1.87 0.85
-
 N-carbamoyl-DL-2-aminohexanoic acid
-
 Achromobacter xylosoxidans
3.5.1.87 0.86
-
 N-carbamoyl-L-leucine
-
 Achromobacter xylosoxidans
3.5.1.87 0.92
-
 N-carbamoyl-L-phenylalanine
-
 Achromobacter xylosoxidans
3.5.1.87 1.5
-
 N-carbamoyl-L-asparagine
-
 Achromobacter xylosoxidans
3.5.1.87 3
-
 N-carbamoyl-L-alanine
-
 Achromobacter xylosoxidans
3.5.1.87 3.2
-
 N-carbamoyl-DL-methionine
-
 Achromobacter xylosoxidans
3.5.1.87 6.6
-
 N-Carbamoylglycine
-
 Achromobacter xylosoxidans
3.5.1.87 10
-
 N-carbamoyl-DL-serine
-
 Achromobacter xylosoxidans
3.5.1.87 16
-
 N-formyl-DL-alanine
-
 Achromobacter xylosoxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.87 Co2+ 0.5 mM, 195% activation of 8-hydroxyquinoline treated enzyme with 38% of initial Achromobacter xylosoxidans
3.5.1.87 Mn2+ 0.3 mM, 360% activation of 8-hydroxyquinoline treated enzyme with 38% of initial activity Achromobacter xylosoxidans
3.5.1.87 Ni2+ 0.3 mM, 200% activation of 8-hydroxyquinoline treated enzyme with 38% of initial activity Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.87 65000
-
 2 * 65000, SDS-PAGE Achromobacter xylosoxidans
3.5.1.87 134000
-
 gel filtration Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.87 Achromobacter xylosoxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.87 DEAE-Sephacel, ammonium sulfate, phenyl-Sepharose CL-4B, Mono Q, Superose 12 HR Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.87 1.22
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.87 N-carbamoyl-DL-2-aminohexanoic acid + H2O 24% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans 2-aminohexanoic acid + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-alanine + H2O
-
 Achromobacter xylosoxidans L-alanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-methionine + H2O
-
 Achromobacter xylosoxidans L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-phenylalanine + H2O 4.5% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-serine + H2O 19% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-serine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-threonine + H2O 8.8% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-threonine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-valine + H2O 28% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-valine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-glycine + H2O 75% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans glycine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-alanine + H2O
-
 Achromobacter xylosoxidans L-alanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-asparagine + H2O 64% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-asparagine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-isoleucine + H2O 5.3% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-isoleucine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-leucine + H2O
-
 Achromobacter xylosoxidans L-leucine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O 12% of activity with N-carbamoyl-L-alanine Achromobacter xylosoxidans L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Achromobacter xylosoxidans L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-phenylalanine + H2O
-
 Achromobacter xylosoxidans L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-valine + H2O
-
 Achromobacter xylosoxidans L-valine + CO2 + NH3
-
 ?
3.5.1.87 N-formyl-DL-alanine + H2O 13% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-alanine + CO2
-
 ?
3.5.1.87 N-formyl-DL-leucine + H2O 5.2% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-leucine + CO2
-
 ?
3.5.1.87 N-formyl-DL-methionine + H2O 5.4% of activity with N-carbamoyl-DL-alanine Achromobacter xylosoxidans L-methionine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.87 dimer 2 * 65000, SDS-PAGE Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.87 35
-
-
 Achromobacter xylosoxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.87 30 35 no loss of activity after 30 min at pH 7.4, 30% activity lost after 30 min at 35°C Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.87 8 8.3 N-carbamoyl-L-valine hydrolysis Achromobacter xylosoxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.87 6 9.5 80% of initial activity after 30 min and 30°C Achromobacter xylosoxidans