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Literature summary extracted from

  • Suzuki, T.; Kitajima, K.; Inoue, S.; Inoue, Y.
    Does an animal peptide N:glycanase have the dual role as an enzyme and a carbohydrate binding protein? (1994), Glycoconj. J., 11, 469-476.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.52 liberated glycan reaction product
-
 Mus musculus
3.5.1.52 additional information no effect: yeast mannan, trimannose Elizabethkingia meningoseptica
3.5.1.52 additional information no effect: free peptides formed during reaction, mannose, alpha-methyl-D-mannoside, 5 mM Na2SO4 Mus musculus
3.5.1.52 additional information no effect: thiol inhibitors, iodoacetamide, N-ethylmaleimide, actinomycete protease inhibitors, leupeptins, chymostatin, pepstatin, Mg2+, Ca2+, Mn2+, EDTA, L-cysteine, phenylmethylsulfonyl fluoride, gamma-D-gluconolactone, yeast mannan, triomannose Prunus amygdalus var. dulcis
3.5.1.52 triomannose
-
 Mus musculus
3.5.1.52 yeast mannan
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.52 0.525
-
 Fetuin glycopeptide
-
 Elizabethkingia meningoseptica
3.5.1.52 1.46
-
 Fetuin glycopeptide
-
 Prunus amygdalus var. dulcis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.52 66800
-
 gel filtration Prunus amygdalus var. dulcis
3.5.1.52 105000
-
 2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.52 Elizabethkingia meningoseptica
-
-
-
3.5.1.52 Mus musculus
-
-
-
3.5.1.52 Oryzias latipes
-
 medaka fish
-
3.5.1.52 Prunus amygdalus var. dulcis
-
 almond
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.52
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.52 bovine fetuin glycopeptide + H2O
-
 Mus musculus ?
-
 ?
3.5.1.52 bovine fetuin glycopeptide + H2O
-
 Elizabethkingia meningoseptica ?
-
 ?
3.5.1.52 fetuin glycopeptide + H2O
-
 Elizabethkingia meningoseptica ?
-
 ?
3.5.1.52 fetuin glycopeptide + H2O
-
 Prunus amygdalus var. dulcis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.52 dimer
-
 Prunus amygdalus var. dulcis
3.5.1.52 dimer 2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids Mus musculus
3.5.1.52 monomer
-
 Elizabethkingia meningoseptica
3.5.1.52 monomer
-
 Prunus amygdalus var. dulcis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.52 7
-
 L-929 PNGase, brain PNGase, kidney PNGase Mus musculus