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Literature summary extracted from

  • Suzuki, T.; Seko, A.; Kitajima, K.; Inoue, Y.; Inoue, S.
    Purification and enzymatic properties of peptide:N-glycanase from C3H mouse-derived L-929 fibroblast cells (1994), J. Biol. Chem., 269, 17611-17618.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.52 DTT 10 mM, absence causes inactivation of L-929 PNGase, suggesting S-H group essential for enzyme activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.52 Cu2+
-
 Mus musculus
3.5.1.52 Fe3+
-
 Mus musculus
3.5.1.52 fucose residue alpha-1-3-linked or alpha-1-6-linked to the proximal GlnNAc residue complete inhibition Mus musculus
3.5.1.52 Glycoasparagine 1 mM causes 86% inhibition Mus musculus
3.5.1.52 liberated glycan reaction product causes 82% inhibition Mus musculus
3.5.1.52 Monoiodoacetic acid 2 mM causes 64% inhibition Mus musculus
3.5.1.52 Monoiodoacetic acid 5 mM causes 82% inhibition of the neutral PNGase M Oryzias latipes
3.5.1.52 Zn2+
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.52 0.114
-
 Fetuin glycopeptide
-
 Mus musculus
3.5.1.52 0.871
-
 asiolo-ovomucoid glycopeptide
-
 Elizabethkingia meningoseptica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.52 cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.52 105000
-
 2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids Mus musculus
3.5.1.52 212000
-
 gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.52 Elizabethkingia meningoseptica
-
-
-
3.5.1.52 Mus musculus
-
-
-
3.5.1.52 Oryzias latipes
-
 medaka fish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.52 460fold Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.52 fibroblast C3H mouse-derived L-929 fibroblast cells Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.52 asiolo-ovomucoid glycopeptide + H2O
-
 Elizabethkingia meningoseptica ?
-
 ?
3.5.1.52 bovine fetuin glycopeptide + H2O
-
 Elizabethkingia meningoseptica ?
-
 ?
3.5.1.52 bovine fetuin glycopeptide + H2O Leu-Asn(Man3Gal3GlcNAc5)-Asp-Ser-Arg Mus musculus ?
-
 ?
3.5.1.52 fetuin glycopeptide + H2O
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.52 dimer 2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.52 30
-
-
 Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.52 25 37
-
 Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.52 37
-
 rapidly reduced stability above 37°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.52 7
-
 L-929 PNGase, brain PNGase, kidney PNGase Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.52 6.8 7
-
 Mus musculus