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Literature summary extracted from

  • Kikuchi, M.; Sakaguchi, K.
    Some enzymatic properties and substrate specificities of peptidoglutaminase-I and II (1973), Agric. Biol. Chem., 37, 1813-1821.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.43 AgNO3
-
 Niallia circulans
3.5.1.43 Hg2+ HgCl2 Niallia circulans
3.5.1.43 N-bromosuccinimide
-
 Niallia circulans
3.5.1.43 sodium lauryl sulfate
-
 Niallia circulans
3.5.1.44 AgNO3
-
 Niallia circulans
3.5.1.44 Hg2+
-
 Niallia circulans
3.5.1.44 N-bromosuccinimide
-
 Niallia circulans
3.5.1.44 sodium lauryl sulfate
-
 Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.43 0.1
-
 N-acetyl-L-Gln
-
 Niallia circulans
3.5.1.43 0.11
-
 benzyloxycarbonyl-L-Gln
-
 Niallia circulans
3.5.1.43 0.12
-
 tert-amyloxycarbonyl-L-Gln-L-Pro
-
 Niallia circulans
3.5.1.43 0.16
-
 L-Tyr-L-Gln
-
 Niallia circulans
3.5.1.43 0.19
-
 L-Pro-L-Gln
-
 Niallia circulans
3.5.1.43 0.21
-
 benzyloxycarbonyl-L-Gln-L-Pro
-
 Niallia circulans
3.5.1.43 0.33
-
 Gly-L-Gln
-
 Niallia circulans
3.5.1.43 0.35
-
 L-Leu-Gly-L-Gln
-
 Niallia circulans
3.5.1.43 1.57
-
 benzyloxycarbonyl-D-Gln
-
 Niallia circulans
3.5.1.43 5
-
 L-Gln
-
 Niallia circulans
3.5.1.43 23.3
-
 L-Leu-Gly-L-Gln
-
 Niallia circulans
3.5.1.44 0.09
-
 L-Lys-L-Gln-Gly
-
 Niallia circulans
3.5.1.44 0.12
-
 tert-amyloxycarbonyl-L-Gln-L-Pro
-
 Niallia circulans
3.5.1.44 0.12
-
 benzyloxycarbonyl-L-Gln-Gly
-
 Niallia circulans
3.5.1.44 0.12
-
 L-Phe-L-Gln-Gly
-
 Niallia circulans
3.5.1.44 0.12
-
 L-Ser-L-Gln-Gly
-
 Niallia circulans
3.5.1.44 0.21
-
 benzyloxycarbonyl-L-Gln-L-Pro
-
 Niallia circulans
3.5.1.44 0.66
-
 L-Gln-Gly
-
 Niallia circulans
3.5.1.44 1.25
-
 benzyloxycarbonyl-L-Gln-Gly methyl ester
-
 Niallia circulans
3.5.1.44 1.57
-
 benzyloxycarbonyl-D-Gln
-
 Niallia circulans
3.5.1.44 5
-
 L-Gln
-
 Niallia circulans
3.5.1.44 10
-
 L-Tyr-L-Gln
-
 Niallia circulans
3.5.1.44 14.3
-
 N-acetyl-L-Gln
-
 Niallia circulans
3.5.1.44 16.7
-
 tert-amyloxycarbonyl-L-Gln-L-Leu-Gly
-
 Niallia circulans
3.5.1.44 18.2
-
 Gly-L-Gln
-
 Niallia circulans
3.5.1.44 22.2
-
 benzyloxycarbonyl-L-Gln
-
 Niallia circulans
3.5.1.44 22.2
-
 L-Pro-L-Gln
-
 Niallia circulans
3.5.1.44 44
-
 L-Ile-L-Gln-L-Asn-L-CysH-L-Pro-L-Leu-Gly-N-acetate
-
 Niallia circulans
3.5.1.44 50
-
 L-Gln
-
 Niallia circulans
3.5.1.44 50
-
 L-His-L-Ser-L-Gln-Gly-L-Thr-L-Phe-L-Thr
-
 Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.44 125000
-
 gel filtration Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.43 Niallia circulans
-
-
-
3.5.1.44 Niallia circulans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.43 benzyloxycarbonyl-D-Gln + H2O
-
 Niallia circulans benzyloxycarbonyl-D-Glu + NH3
-
 ?
3.5.1.43 benzyloxycarbonyl-L-Gln + H2O
-
 Niallia circulans benzyloxycarbonyl-L-Glu + NH3
-
 ?
3.5.1.43 benzyloxycarbonyl-L-Gln-Gly + H2O peptidoglutaminase II Niallia circulans benzyloxycarbonyl-L-Glu-Gly + NH3
-
 ?
3.5.1.43 benzyloxycarbonyl-L-Gln-Gly methyl ester + H2O peptidoglutaminase II Niallia circulans benzyloxycarbonyl-L-Glu-Gly methyl ester + NH3
-
 ?
3.5.1.43 benzyloxycarbonyl-L-Gln-L-Pro + H2O peptidoglutaminase II Niallia circulans benzyloxycarbonyl-L-Glu-L-Pro + NH3
-
 ?
3.5.1.43 Gly-L-Gln + H2O
-
 Niallia circulans Gly-L-Glu + NH3
-
 ?
3.5.1.43 Gly-L-Pro-L-Leu-Gly-L-Gln + H2O
-
 Niallia circulans Gly-L-Pro-L-Leu-Gly-L-Glu + NH3
-
 ?
3.5.1.43 L-Gln + H2O
-
 Niallia circulans L-Glu + NH3
-
 ?
3.5.1.43 L-Gln-Gly + H2O + H2O peptidoglutaminase II Niallia circulans L-Glu-Gly + NH3
-
 ?
3.5.1.43 L-His-L-Ser-L-Gln-Gly-L-Thr-L-Phe-L-Thr + H2O
-
 Niallia circulans L-His-L-Ser-L-Glu-Gly-L-Thr-L-Phe-L-Thr + NH3
-
 ?
3.5.1.43 L-Ile-L-Gln-L-Asn-L-CysH-L-Pro-L-Leu-Gly-acetate + H2O
-
 Niallia circulans L-Ile-L-Glu-L-Asn-L-CysH-L-Pro-L-Leu-Gly-acetate + NH3
-
 ?
3.5.1.43 L-Leu-Gly-L-Gln + H2O
-
 Niallia circulans L-Leu-Gly-L-Glu + NH3
-
 ?
3.5.1.43 L-Lys-L-Gln-Gly + H2O
-
 Niallia circulans L-Lys-L-Glu-Gly + NH3
-
 ?
3.5.1.43 L-Phe-L-Gln-Gly + H2O
-
 Niallia circulans L-Phe-L-Glu-Gly + NH3
-
 ?
3.5.1.43 L-Pro-L-Gln + H2O
-
 Niallia circulans L-Pro-L-Glu + NH3
-
 ?
3.5.1.43 L-Pro-L-Leu-Gly-L-Gln + H2O
-
 Niallia circulans L-Pro-L-Leu-Gly-L-Glu + NH3
-
 ?
3.5.1.43 L-Ser-L-Gln-Gly + H2O
-
 Niallia circulans L-Ser-L-Glu-Gly + NH3
-
 ?
3.5.1.43 L-Tyr-L-Gln + H2O
-
 Niallia circulans L-Tyr-L-Glu + NH3
-
 ?
3.5.1.43 N-acetyl-L-Gln + H2O
-
 Niallia circulans N-acetyl-L-Glu + NH3
-
 ?
3.5.1.43 tert-amyloxycarbonyl-L-Gln-L-Leu-Gly + H2O
-
 Niallia circulans tert-amyloxycarbonyl-L-Glu-L-Leu-Gly + NH3
-
 ?
3.5.1.43 tert-amyloxycarbonyl-L-Gln-L-Pro + H2O
-
 Niallia circulans tert-amyloxycarbonyl-L-Glu-Pro + NH3
-
 ?
3.5.1.43 tert-amyloxycarbonyl-L-Gln-Pro + H2O peptidoglutaminase II Niallia circulans tert-amyloxycarbonyl-L-Glu-L-Leu-Gly + NH3
-
 ?
3.5.1.44 benzyloxycarbonyl-D-Gln + H2O
-
 Niallia circulans ?
-
 ?
3.5.1.44 benzyloxycarbonyl-L-Gln + H2O
-
 Niallia circulans benzyloxycarbonyl-L-Glu + NH3
-
 ?
3.5.1.44 benzyloxycarbonyl-L-Gln-Gly + H2O
-
 Niallia circulans benzyloxycarbonyl-L-Glu-Gly + NH3
-
 ?
3.5.1.44 benzyloxycarbonyl-L-Gln-Gly methyl ester + H2O
-
 Niallia circulans benzyloxycarbonyl-L-Glu-Gly methyl ester + NH3
-
 ?
3.5.1.44 benzyloxycarbonyl-L-Gln-L-Pro + H2O
-
 Niallia circulans benzyloxycarbonyl-L-Glu-L-Pro + NH3
-
 ?
3.5.1.44 Gly-L-Gln + H2O
-
 Niallia circulans Gly-L-Glu + NH3
-
 ?
3.5.1.44 insulin + H2O two L-Gln in oxidized insulin A chain are attacked Niallia circulans NH3 + ?
-
 ?
3.5.1.44 L-Gln + H2O
-
 Niallia circulans L-Glu + NH3
-
 ?
3.5.1.44 L-Gln-Gly + H2O
-
 Niallia circulans L-Glu-Gly + NH3
-
 ?
3.5.1.44 L-His-L-Ser-L-Gln-Gly-L-Thr-L-Phe-L-Thr + H2O
-
 Niallia circulans L-His-L-Ser-L-Glu-Gly-L-Thr-L-Phe-L-Thr + NH3
-
 ?
3.5.1.44 L-Ile-L-Gln-L-Asn-L-Cys-L-Pro-L-Leu-Gly-NH2 + H2O
-
 Niallia circulans L-Ile-L-Gln-L-Asn-L-Cys-L-Pro-L-Leu-Gly + NH3
-
 ?
3.5.1.44 L-Ile-L-Gln-L-Asn-L-CysH-L-Pro-L-Leu-Gly-N-acetate + H2O
-
 Niallia circulans ?
-
 ?
3.5.1.44 L-Leu-Gly-L-Gln + H2O
-
 Niallia circulans L-Leu-Gly-L-Gln + NH3
-
 ?
3.5.1.44 L-Lys-L-Gln-Gly + H2O
-
 Niallia circulans L-Lys-L-Glu-Gly + NH3
-
 ?
3.5.1.44 L-Phe-L-Gln-Gly + H2O
-
 Niallia circulans L-Phe-L-Glu-Gly + NH3
-
 ?
3.5.1.44 L-Pro-L-Gln + H2O
-
 Niallia circulans L-Pro-L-Glu + NH3
-
 ?
3.5.1.44 L-Ser-L-Gln-Gly + H2O
-
 Niallia circulans L-Ser-L-Glu-Gly + NH3
-
 ?
3.5.1.44 L-Tyr-L-Gln + H2O
-
 Niallia circulans L-Tyr-L-Glu + NH3
-
 ?
3.5.1.44 N-acetyl-L-Gln + H2O
-
 Niallia circulans N-acetyl-L-Glu + NH3
-
 ?
3.5.1.44 tert-amyloxycarbonyl-L-Gln-L-Leu-Gly + H2O
-
 Niallia circulans tert-amyloxycarbonyl-L-Gln-L-Leu-Gly + NH3
-
 ?
3.5.1.44 tert-amyloxycarbonyl-L-Gln-L-Pro + H2O
-
 Niallia circulans tert-amyloxycarbonyl-L-Glu-Pro + NH3
-
 ?
3.5.1.44 tert-amyloxycarbonyl-L-Gln-Pro + H2O
-
 Niallia circulans tert-amyloxycarbonyl-L-Glu-Pro + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.43 55
-
 hydrolysis of benzyloxycarbonyl-L-Gln Niallia circulans
3.5.1.44 50
-
 hydrolysis of tert-amyloxycarbonyl-L-Gln-L-Pro Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.43 40
-
 10 min, stable up to Niallia circulans
3.5.1.43 55
-
 10 min, complete inactivation Niallia circulans
3.5.1.44 40
-
 10 min, stable up to Niallia circulans
3.5.1.44 50
-
 10 min, complete inactivation Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.43 8
-
 hydrolysis of benzyloxycarbonyl-L-Gln Niallia circulans
3.5.1.44 8
-
 hydrolysis of tert-amyloxycarbonyl-L-Gln-L-Pro Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.43 5 12.5 40°C, 1 h, stable Niallia circulans
3.5.1.43 5 12.5 1°C, 22 h, stable Niallia circulans
3.5.1.44 6 8 40°C, 1 h, stable Niallia circulans
3.5.1.44 6 11 1°C, 22 h, stable Niallia circulans