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Literature summary extracted from

  • Ramaley, R.F.; Fernald, N.; DeVries, T.
    Dicarboxylate omega-amidase of Bacillus subtilis-168: evidence for a membrane-associated form (1972), Arch. Biochem. Biophys., 153, 88-94.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.3 sodium deoxycholate membrane-associated form Bacillus subtilis
3.5.1.3 sodium lauryl sulfate membrane-associated form Bacillus subtilis
3.5.1.3 Triton X-100 membrane-associated form Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.3 NH4+ biosynthesis of omega-amidase is repressed by free ammonium ions; biosynthesis of omega-amidase strongly repressed by ammonia, 0.001 M: 98% repression, 0.005 M: complete repression, ammonia has no effect on catalytic activity: succinyl hydroxamate formation Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.3 cytoplasm
-
 Bacillus subtilis 5737
-
3.5.1.3 membrane membrane-associated, one third to one fourth of total omega-amidase Bacillus subtilis 16020
-
3.5.1.3 membrane not covalently bound to membrane Bacillus subtilis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.3 Bacillus subtilis
-
 168, wild-type
-
3.5.1.3 Bacillus subtilis 168
-
 168, wild-type
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.3 partial, membrane-associated, with detergents Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.3 0.59
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.3 succinamate + hydroxylamine
-
 Bacillus subtilis succinyl hydroxamate + NH3
-
 r
3.5.1.3 succinamate + hydroxylamine
-
 Bacillus subtilis 168 succinyl hydroxamate + NH3
-
 r
3.5.1.3 succinate + hydroxylamine
-
 Bacillus subtilis succinyl hydroxamate + H2O
-
 r
3.5.1.3 succinate + hydroxylamine
-
 Bacillus subtilis 168 succinyl hydroxamate + H2O
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.3 37
-
 assay at Bacillus subtilis