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Literature summary extracted from

  • Yang, Y.B.; Hu, H.L.; Chang, M.C.; Li, H.; Tsai, Y.C.
    Purification and characterization of L-aminoacylase from Alcaligenes denitrificans DA181 (1994), Biosci. Biotechnol. Biochem., 58, 204-205.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.14 synthesis
-
 Sus scrofa
3.5.1.14 synthesis
-
 Bacillus sp. (in: Bacteria)
3.5.1.14 synthesis
-
 Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 Cd2+
-
 Alcaligenes sp.
3.5.1.14 Cd2+
-
 Aspergillus oryzae
3.5.1.14 Cd2+
-
 Bacillus sp. (in: Bacteria)
3.5.1.14 Cu2+
-
 Alcaligenes sp.
3.5.1.14 Cu2+
-
 Aspergillus oryzae
3.5.1.14 Cu2+
-
 Bacillus sp. (in: Bacteria)
3.5.1.14 EDTA
-
 Alcaligenes sp.
3.5.1.14 EDTA
-
 Aspergillus oryzae
3.5.1.14 Pb2+
-
 Alcaligenes sp.
3.5.1.14 Zn2+
-
 Alcaligenes sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.14 intracellular
-
 Alcaligenes sp. 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Sus scrofa
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Bacillus sp. (in: Bacteria)
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Alcaligenes sp.
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Aspergillus oryzae
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Sus scrofa
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Bacillus sp. (in: Bacteria)
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Alcaligenes sp.
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 40000
-
 2 * 40000, SDS-PAGE Alcaligenes sp.
3.5.1.14 74000
-
-
 Aspergillus oryzae
3.5.1.14 80000
-
 gel filtration Alcaligenes sp.
3.5.1.14 175000
-
-
 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Bacillus sp. (in: Bacteria)
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Alcaligenes sp.
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Aspergillus oryzae No.9
-
 L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Alcaligenes sp.
-
 denitrificans DA181, M14
-
3.5.1.14 Alcaligenes sp.
-
 xylosoxydans subsp. xylosoxydans A-6
-
3.5.1.14 Aspergillus oryzae
-
 No.9
-
3.5.1.14 Aspergillus oryzae No.9
-
 No.9
-
3.5.1.14 Bacillus sp. (in: Bacteria)
-
 thermoglucosidicus, strain DSM 2542
-
3.5.1.14 Cercidium texanum
-
 paloverde tree
-
3.5.1.14 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14
-
 Alcaligenes sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.14 seed
-
 Cercidium texanum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Bacillus sp. (in: Bacteria) DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Alcaligenes sp. DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae No.9 DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Sus scrofa L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Bacillus sp. (in: Bacteria) L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Alcaligenes sp. L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae No.9 L-alanine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Bacillus sp. (in: Bacteria) L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Alcaligenes sp. L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Aspergillus oryzae No.9 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Sus scrofa L-valine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Bacillus sp. (in: Bacteria) L-valine + acetate
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O favorite substrate Alcaligenes sp. L-valine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 dimer
-
 Sus scrofa
3.5.1.14 dimer
-
 Aspergillus oryzae
3.5.1.14 dimer 2 * 40000, SDS-PAGE Alcaligenes sp.
3.5.1.14 tetramer
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 65
-
-
 Sus scrofa
3.5.1.14 65
-
-
 Alcaligenes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.14 60
-
-
 Sus scrofa
3.5.1.14 60
-
-
 Aspergillus oryzae
3.5.1.14 60
-
 retains 80% activity after 1 h at 60°C Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 8
-
-
 Sus scrofa
3.5.1.14 8
-
-
 Alcaligenes sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.14 4 6.5
-
 Alcaligenes sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.14 5 9
-
 Alcaligenes sp.