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Literature summary extracted from

  • Henseling, J.; Roehm, K.H.
    Unusual solvent isotope effects on the aminoacylase-catalyzed hydrolysis of acetylamino acids (1987), FEBS Lett., 219, 27-30.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.14 analysis enzyme widely used as reagent to resolve amino acid racemates Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.99
-
N-acetyl-L-methionine
-
Sus scrofa
3.5.1.14 1.62
-
N-acetyl-L-methionine ordinary water, 50 mM MOPS 50 mM bicine buffer Sus scrofa
3.5.1.14 1.87
-
N-acetyl-L-methionine hydrolysis in D2O, 50 mM MOPS 50 mM bicine buffer Sus scrofa
3.5.1.14 5.5
-
N-acetyl-L-phenylalanine ordinary water, MOPS/NaOH buffer Sus scrofa
3.5.1.14 5.6
-
N-acetyl-L-phenylalanine hydrolysis in D2O, MOPS/NaOH buffer, distinctly inverse deuterium isotope effect Sus scrofa
3.5.1.14 7
-
N-acetyl-L-glutamine hydrolysis in D2O, MOPS/NaOH buffer Sus scrofa
3.5.1.14 10.2
-
N-acetyl-L-glutamine MOPS/NaOH buffer Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Sus scrofa
-
L-methionine + acetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Sus scrofa
-
pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14
-
Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.14 kidney
-
Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-DL-methionine + H2O
-
Sus scrofa DL-methionine + acetate
-
?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
Sus scrofa L-glutamine + acetate
-
?
3.5.1.14 N-acetyl-L-methionine
-
Sus scrofa L-methionine + acetate
-
?
3.5.1.14 N-acetyl-L-phenylalanine + H2O
-
Sus scrofa L-phenylalanine + acetate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 hog kidney aminoacylase I
-
Sus scrofa