The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity
Beylot, M.H.; McKie, V.A.; Voragen, A.G.J.; Doeswijk-Voragen, C.H.L.; Gilbert, H.J.; Biochem. J. 358, 607-614 (2001) 
Data extracted from this reference:
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.55
gene abf51A amplified by PCR
Cellvibrio japonicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.55
0.042
-
p-nitrophenyl-alpha-L-arabinofuranoside
mutant E194A
Cellvibrio japonicus
3.2.1.55
0.18
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Cellvibrio japonicus
3.2.1.55
0.26
-
4-methylumbelliferyl-alpha-L-arabinofuranoside
-
Cellvibrio japonicus
3.2.1.55
0.27
-
wheat arabinoxylan
-
Cellvibrio japonicus
3.2.1.55
0.33
-
sugar beet arabinan
-
Cellvibrio japonicus
3.2.1.55
1.58
-
arabinobiose
-
Cellvibrio japonicus
3.2.1.55
1.7
-
arabinobiose
-
Cellvibrio japonicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.55
57000
-
SDS-PAGE
Cellvibrio japonicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.55
beet arabinan + H2O
Streptomyces chartreusis
-
L-arabinose + ?
-
-
ir
3.2.1.55
beet arabinan + H2O
Cellvibrio japonicus
-
L-arabinose + ?
-
-
ir
3.2.1.55
wheat L-arabino-D-xylan + H2O
Cellvibrio japonicus
-
L-arabinose + ?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.55
Bifidobacterium adolescentis
-
-
-
3.2.1.55
Cellvibrio japonicus
-
-
-
3.2.1.55
Pseudomonas fluorescens
-
-
-
3.2.1.55
Streptomyces chartreusis
-
GS901
-
3.2.1.55
Streptomyces chartreusis GS901
-
GS901
-
3.2.1.55
Thermoclostridium stercorarium
O08457
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.55
1,5-alpha-L-arabinobiose + H2O
-
288848
Cellvibrio japonicus
L-arabinose
-
-
-
?
3.2.1.55
1,5-alpha-L-arabinofuranohexaose + H2O
-
288848
Cellvibrio japonicus
L-arabinose + L-arabinopentaose
-
-
-
?
3.2.1.55
1,5-alpha-L-arabinotetraose + H2O
-
288848
Cellvibrio japonicus
L-arabinose
-
-
-
?
3.2.1.55
1,5-alpha-L-arabinotriose + H2O
-
288848
Cellvibrio japonicus
L-arabinose
-
-
-
?
3.2.1.55
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis GS901
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
3-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O
-
288848
Cellvibrio japonicus
methylumbelliferone + alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
beet arabinan + H2O
-
288848
Streptomyces chartreusis
L-arabinose + ?
-
-
-
ir
3.2.1.55
beet arabinan + H2O
-
288848
Cellvibrio japonicus
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinan + H2O
-
288848
Streptomyces chartreusis
(1,5-alpha-L-arabinose)25 + ?
-
-
-
?
3.2.1.55
L-arabinan + H2O
preferentially removes alpha 1,2 and alpha 1,3-linked arabinofuranose side chains
288848
Cellvibrio japonicus
(1,5-alpha-L-arabinose)25 + ?
-
-
-
?
3.2.1.55
L-arabinan + H2O
-
288848
Streptomyces chartreusis GS901
(1,5-alpha-L-arabinose)25 + ?
-
-
-
?
3.2.1.55
L-arabinopentaose + H2O
-
288848
Cellvibrio japonicus
L-arabinotetraose + L-arabinose
-
-
-
?
3.2.1.55
L-arabinoxylan + H2O
-
288848
Bifidobacterium adolescentis
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
-
288848
Streptomyces chartreusis
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
-
288848
Thermoclostridium stercorarium
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
preferentially removes alpha-1,2- and alpha-1,3-linked arabinofuranose side chains
288848
Cellvibrio japonicus
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
-
288848
Streptomyces chartreusis GS901
L-arabinose + ?
-
-
-
ir
3.2.1.55
methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
288848
Cellvibrio japonicus
methylumbelliferone
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Cellvibrio japonicus
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Thermoclostridium stercorarium
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis GS901
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Pseudomonas fluorescens
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Bifidobacterium adolescentis
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Cellvibrio japonicus
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Thermoclostridium stercorarium
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis GS901
L-arabinose + phenol
-
-
-
ir
3.2.1.55
sugar beet arabinan + H2O
-
288848
Cellvibrio japonicus
L-arabinofuranose + ?
-
-
-
?
3.2.1.55
wheat arabinoxylan + H2O
-
288848
Cellvibrio japonicus
L-arabinofuranose + ?
-
-
-
?
3.2.1.55
wheat L-arabino-D-xylan + H2O
-
288848
Cellvibrio japonicus
L-arabinose + ?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.55
5.5
-
-
Cellvibrio japonicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.55
gene abf51A amplified by PCR
Cellvibrio japonicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.55
0.042
-
p-nitrophenyl-alpha-L-arabinofuranoside
mutant E194A
Cellvibrio japonicus
3.2.1.55
0.18
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Cellvibrio japonicus
3.2.1.55
0.26
-
4-methylumbelliferyl-alpha-L-arabinofuranoside
-
Cellvibrio japonicus
3.2.1.55
0.27
-
wheat arabinoxylan
-
Cellvibrio japonicus
3.2.1.55
0.33
-
sugar beet arabinan
-
Cellvibrio japonicus
3.2.1.55
1.58
-
arabinobiose
-
Cellvibrio japonicus
3.2.1.55
1.7
-
arabinobiose
-
Cellvibrio japonicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.55
57000
-
SDS-PAGE
Cellvibrio japonicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.55
beet arabinan + H2O
Streptomyces chartreusis
-
L-arabinose + ?
-
-
ir
3.2.1.55
beet arabinan + H2O
Cellvibrio japonicus
-
L-arabinose + ?
-
-
ir
3.2.1.55
wheat L-arabino-D-xylan + H2O
Cellvibrio japonicus
-
L-arabinose + ?
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.55
1,5-alpha-L-arabinobiose + H2O
-
288848
Cellvibrio japonicus
L-arabinose
-
-
-
?
3.2.1.55
1,5-alpha-L-arabinofuranohexaose + H2O
-
288848
Cellvibrio japonicus
L-arabinose + L-arabinopentaose
-
-
-
?
3.2.1.55
1,5-alpha-L-arabinotetraose + H2O
-
288848
Cellvibrio japonicus
L-arabinose
-
-
-
?
3.2.1.55
1,5-alpha-L-arabinotriose + H2O
-
288848
Cellvibrio japonicus
L-arabinose
-
-
-
?
3.2.1.55
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis GS901
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
3-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O
-
288848
Cellvibrio japonicus
methylumbelliferone + alpha-L-arabinofuranose
-
-
-
?
3.2.1.55
beet arabinan + H2O
-
288848
Streptomyces chartreusis
L-arabinose + ?
-
-
-
ir
3.2.1.55
beet arabinan + H2O
-
288848
Cellvibrio japonicus
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinan + H2O
-
288848
Streptomyces chartreusis
(1,5-alpha-L-arabinose)25 + ?
-
-
-
?
3.2.1.55
L-arabinan + H2O
preferentially removes alpha 1,2 and alpha 1,3-linked arabinofuranose side chains
288848
Cellvibrio japonicus
(1,5-alpha-L-arabinose)25 + ?
-
-
-
?
3.2.1.55
L-arabinan + H2O
-
288848
Streptomyces chartreusis GS901
(1,5-alpha-L-arabinose)25 + ?
-
-
-
?
3.2.1.55
L-arabinopentaose + H2O
-
288848
Cellvibrio japonicus
L-arabinotetraose + L-arabinose
-
-
-
?
3.2.1.55
L-arabinoxylan + H2O
-
288848
Bifidobacterium adolescentis
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
-
288848
Streptomyces chartreusis
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
-
288848
Thermoclostridium stercorarium
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
preferentially removes alpha-1,2- and alpha-1,3-linked arabinofuranose side chains
288848
Cellvibrio japonicus
L-arabinose + ?
-
-
-
ir
3.2.1.55
L-arabinoxylan + H2O
-
288848
Streptomyces chartreusis GS901
L-arabinose + ?
-
-
-
ir
3.2.1.55
methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
288848
Cellvibrio japonicus
methylumbelliferone
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Cellvibrio japonicus
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Thermoclostridium stercorarium
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis GS901
p-nitrophenol + L-arabinose
-
-
-
?
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Pseudomonas fluorescens
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Bifidobacterium adolescentis
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Cellvibrio japonicus
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Thermoclostridium stercorarium
L-arabinose + phenol
-
-
-
ir
3.2.1.55
phenyl alpha-L-arabinofuranoside + H2O
-
288848
Streptomyces chartreusis GS901
L-arabinose + phenol
-
-
-
ir
3.2.1.55
sugar beet arabinan + H2O
-
288848
Cellvibrio japonicus
L-arabinofuranose + ?
-
-
-
?
3.2.1.55
wheat arabinoxylan + H2O
-
288848
Cellvibrio japonicus
L-arabinofuranose + ?
-
-
-
?
3.2.1.55
wheat L-arabino-D-xylan + H2O
-
288848
Cellvibrio japonicus
L-arabinose + ?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.55
5.5
-
-
Cellvibrio japonicus