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Literature summary extracted from

  • Lee, R.C.; Burton, R.A.; Hrmova, M.; Fincher, G.B.
    Barley arabinoxylan arabinofuranohydrolases: Purification, characterization and determination of primary structures from cDNA clones (2001), Biochem. J., 356, 181-189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 cDNA clones isolated Hordeum vulgare
3.2.1.55 cDNA isolation Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.77
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 65000
-
-
 Bacillus subtilis
3.2.1.55 65000
-
 SDS-PAGE Hordeum vulgare
3.2.1.55 68280
-
 isoenzyme AXAH, encoded by cDNA Hordeum vulgare
3.2.1.55 68760
-
 isoenzyme AXAH-II, encoded by cDNA Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 beet arabinan + H2O Bacillus subtilis
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Geobacillus stearothermophilus
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Hordeum vulgare
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Streptomyces chartreusis
-
 L-arabinose + ?
-
 ir
3.2.1.55 larchwood arabinogalactan + H2O Hordeum vulgare
-
 L-arabinose + ?
-
 ?
3.2.1.55 sugar beet arabinan + H2O Hordeum vulgare
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat-flour arabinoxylan + H2O Hordeum vulgare
-
 L-arabinofuranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Arabidopsis thaliana
-
-
-
3.2.1.55 Aspergillus niger P42254
-
-
3.2.1.55 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.55 Bacillus subtilis
-
-
-
3.2.1.55 Bacteroides ovatus Q59218
-
-
3.2.1.55 Breznakibacter xylanolyticus
-
-
-
3.2.1.55 Geobacillus stearothermophilus
-
-
-
3.2.1.55 Hordeum vulgare
-
 L.cv. Clipper
-
3.2.1.55 Streptomyces chartreusis P82593
-
-
3.2.1.55 Streptomyces coelicolor
-
-
-
3.2.1.55 Thermoclostridium stercorarium O08457
-
-
3.2.1.55 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Hordeum vulgare
3.2.1.55
-
 Streptomyces chartreusis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 caryopsis
-
 Hordeum vulgare
-
3.2.1.55 seedling
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 6.3
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-alpha-L-arabinofuranohexaose + H2O
-
 Hordeum vulgare L-arabinose + L-arabinopentaose
-
 ?
3.2.1.55 1,5-alpha-L-arabinofuranohexaose + H2O
-
 Aspergillus niger L-arabinose + L-arabinopentaose
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Bacillus subtilis L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Geobacillus stearothermophilus L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Streptomyces chartreusis L-arabinose + ?
-
 ir
3.2.1.55 L-arabinan + H2O
-
 Bacillus subtilis (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Geobacillus stearothermophilus (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Hordeum vulgare (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces chartreusis (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Geobacillus stearothermophilus L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Streptomyces chartreusis L-arabinose + ?
-
 ?
3.2.1.55 L-arabinoxylan + H2O
-
 Geobacillus stearothermophilus L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces chartreusis L-arabinose + ?
-
 ir
3.2.1.55 larchwood arabinogalactan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ?
3.2.1.55 additional information
-
 Bacillus subtilis ?
-
 ?
3.2.1.55 additional information
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces chartreusis ?
-
 ?
3.2.1.55 additional information enzyme is unable to hydrolyse 4-nitrophenyl arabinopyranosides, xylopyranosides, mannopyranosides, glucopyranosides or galactopyranosides, enzyme does not hydrolyse laminarin, beta-glucan, gum arabic or carboxymethylxylan Hordeum vulgare ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Arabidopsis thaliana L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus sp. (in: Bacteria) L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces coelicolor L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Thermotoga maritima L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Breznakibacter xylanolyticus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bacteroides ovatus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis L-arabinose + phenol
-
 ir
3.2.1.55 sugar beet arabinan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ?
3.2.1.55 wheat-flour arabinoxylan + H2O
-
 Hordeum vulgare L-arabinofuranose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 arabinoxylan arabinofuranohydrolase
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4.3
-
-
 Hordeum vulgare