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Literature summary extracted from

  • Hirano, Y.; Tsumuraya, Y.; Hashimoto, Y.
    Characterization of spinach leaf alpha-L-arabinofuranosidases and beta-galactosidases and their synergistic action on an endogenous arabinogalactan-protein (1994), Physiol. Plant., 92, 286-296.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Ag+ alpha-L-arafase I Spinacia oleracea
3.2.1.55 D-glucono-1,5-lactone alpha-L-arafase I Spinacia oleracea
3.2.1.55 D-xylose alpha-arafase I Spinacia oleracea
3.2.1.55 Hg2+ alpha-L-arafase I Spinacia oleracea
3.2.1.55 SDS alpha-L-arafase I Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 1.2
-
 p-nitrophenyl-alpha-L-arabinofuranoside alpha-L-arafase I Spinacia oleracea
3.2.1.55 2.2
-
 p-nitrophenyl-alpha-L-arabinofuranoside alpha-L-arafase II Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 cell membrane
-
 Spinacia oleracea
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 68000
-
 alpha-L-arafase II Spinacia oleracea
3.2.1.55 118000
-
 alpha-L-arafase I Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 sugar beet arabinan + H2O Spinacia oleracea alpha-L-arafase II L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Raphanus sativus
-
 radish
-
3.2.1.55 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 2 isoenzymes, alpha-L-arafase I and alpha-L-arafase II Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 seed
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 0.61
-
 alpha-L-arafase I Spinacia oleracea
3.2.1.55 2.5
-
 alpha-L-arafase II Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Spinacia oleracea p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-beta-D-xylopyranoside + H2O alpha-L-arafase I Spinacia oleracea 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 sugar beet arabinan + H2O alpha-L-arafase II Spinacia oleracea L-arabinose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arafase I
-
 Spinacia oleracea
3.2.1.55 alpha-L-arafase II
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4.8
-
-
 Spinacia oleracea