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Literature summary extracted from

  • Tagawa, K.; Kaji, A.
    alpha-L-arabinofuranosidase from Aspergillus niger (1988), Methods Enzymol., 160, 707-712.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.55
-
 Aspergillus niger

General Stability

EC Number General Stability Organism
3.2.1.55 completely denatured in 6 M urea Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Ag+ inhibited by AgNO3 Aspergillus niger
3.2.1.55 L-arabinose competitive inhibitor Aspergillus niger
3.2.1.55 mercurous acetate
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 5
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Aspergillus niger
3.2.1.55 5
-
 phenyl-alpha-L-arabinofuranoside
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 53000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 gum arabic + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 388.6
-
-
 Aspergillus niger
3.2.1.55 390
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
3.2.1.55 5°C, if aseptically stored no significant loss of activity over a 3-months period Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 gum arabic + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 3.8
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 2 5.5
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 2.5 8.5 considerable stability Aspergillus niger
3.2.1.55 7
-
 extremely stable at Aspergillus niger