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Literature summary extracted from

  • Komae, K.; Kaji, A.; Sato, M.
    An alpha-L-arabinofuranosidase from Streptomyces purpurascens IFO 3389 (1982), Agric. Biol. Chem., 46, 1899-1905.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.55
-
 Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Ag+
-
 Aspergillus niger
3.2.1.55 Ag+
-
 Rhodotorula flava
3.2.1.55 Ag+
-
 Streptomyces purpurascens
3.2.1.55 Hg2+
-
 Pectobacterium carotovorum
3.2.1.55 Hg2+
-
 Rhodotorula flava
3.2.1.55 Hg2+
-
 Streptomyces purpurascens
3.2.1.55 L-arabino-gamma-lactone
-
 Streptomyces purpurascens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.082
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Streptomyces purpurascens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 62000
-
 8 * 62000 Streptomyces purpurascens
3.2.1.55 495000
-
 gel filtration Streptomyces purpurascens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 beet arabinan + H2O Bacillus subtilis
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Pectobacterium carotovorum
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Athelia rolfsii
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Rhodotorula flava
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Streptomyces purpurascens
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Albifimbria verrucaria
-
-
-
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Athelia rolfsii
-
-
-
3.2.1.55 Bacillus subtilis
-
-
-
3.2.1.55 Botrytis cinerea
-
-
-
3.2.1.55 Botrytis fabae
-
-
-
3.2.1.55 Clostridium felsineum
-
-
-
3.2.1.55 Kitasatospora aureofaciens
-
-
-
3.2.1.55 Kitasatospora aureofaciens KA 204
-
-
-
3.2.1.55 Pectobacterium carotovorum
-
-
-
3.2.1.55 Rhodotorula flava
-
-
-
3.2.1.55 Sclerotinia fructigena
-
-
-
3.2.1.55 Sclerotinia libertiana
-
-
-
3.2.1.55 Streptomyces flaveolus
-
-
-
3.2.1.55 Streptomyces flaveolus KA1143
-
-
-
3.2.1.55 Streptomyces lavendulae
-
-
-
3.2.1.55 Streptomyces lavendulae KA140
-
-
-
3.2.1.55 Streptomyces massasporeus
-
 IFO 3841
-
3.2.1.55 Streptomyces olivochromogenes
-
 IFO3178
-
3.2.1.55 Streptomyces olivochromogenes IFO3178
-
 IFO3178
-
3.2.1.55 Streptomyces purpurascens
-
 IFO 3389
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Aspergillus niger
3.2.1.55
-
 Streptomyces purpurascens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 82.9
-
-
 Streptomyces purpurascens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 beet arabinan + H2O
-
 Bacillus subtilis L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Pectobacterium carotovorum L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Athelia rolfsii L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Rhodotorula flava L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Streptomyces purpurascens L-arabinose + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Bacillus subtilis (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces olivochromogenes (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Kitasatospora aureofaciens (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Albifimbria verrucaria (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces lavendulae (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces purpurascens (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces flaveolus (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Botrytis fabae (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces lavendulae KA140 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces olivochromogenes IFO3178 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Kitasatospora aureofaciens KA 204 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces flaveolus KA1143 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 additional information
-
 Bacillus subtilis ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information
-
 Pectobacterium carotovorum ?
-
 ?
3.2.1.55 additional information
-
 Rhodotorula flava ?
-
 ?
3.2.1.55 additional information inactive towards arabinan, arabinoxylan and arabinogalactan Streptomyces purpurascens ?
-
 ?
3.2.1.55 additional information inactive on p-nitrophenyl beta-D-galactopyranoside, phenyl beta-L-arabinopyranosideand p-nitrophenyl glycosides like alpha-D-galactopyranoside, alpha-and beta-D-glucopyranosides, alpha- and beta-D-xylopyranosides, alpha-L-fucopyranoside, alpha-D-mannopyranoside and N-acetyl beta-D-glucosaminide Streptomyces purpurascens ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens p-nitrophenol + L-arabinose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 octamer 8 * 62000 Streptomyces purpurascens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 6.5
-
-
 Bacillus subtilis
3.2.1.55 6.5
-
-
 Streptomyces purpurascens