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Literature summary extracted from
- The preparation of lactase and glucoamylase of rat small intestine (1972), Biochim. Biophys. Acta, 258, 506-519.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.108 | 21 | - |
lactose | - |
Homo sapiens |  |
| 3.2.1.108 | 21 | - |
lactose | - |
Rattus norvegicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.108 | Homo sapiens | - |
- |
- |
| 3.2.1.108 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.108 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.108 | small intestine | - |
Homo sapiens | - |
| 3.2.1.108 | small intestine | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.108 | 22 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.108 | -20°C, stable for several months | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.108 | lactose + H2O | - |
Homo sapiens | D-glucose + D-galactose | - |
? | |
| 3.2.1.108 | lactose + H2O | - |
Rattus norvegicus | D-glucose + D-galactose | - |
? | |
| 3.2.1.108 | phlorizin + H2O | - |
Homo sapiens | phloretin + D-glucose | - |
? | |
| 3.2.1.108 | phlorizin + H2O | - |
Rattus norvegicus | phloretin + D-glucose | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.108 | 37 | - |
about 30% loss of lactase activity after 30 min | Rattus norvegicus |
| 3.2.1.108 | 46 | - |
35 min, about 50% loss of phlorizin hydrolase activity. About 70% loss of lactase activity after 25 min | Rattus norvegicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.108 | 5.5 | - |
30% loss of activity after 0.5 min, 55% loss of activity after 120 min | Rattus norvegicus |
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Release 2023.1 (January 2023)
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