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Literature summary extracted from

  • Ikai, H.; Yamamoto, S.
    Identification and analysis of a gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase involved in the 1,3-diaminopropane production pathway in Acinetobacter baumannii (1997), J. Bacteriol., 179, 5118-5125.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter calcoaceticus
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter johnsonii
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter lwoffii
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter baumannii
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter genospecies
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter haemolyticus
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter junii
2.6.1.76 medicine drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics Acinetobacter radioresistens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.76 homologs of dat detected by PCR amplification Acinetobacter calcoaceticus
2.6.1.76 homologs of dat detected by PCR amplification Acinetobacter johnsonii
2.6.1.76 homologs of dat detected by PCR amplification Acinetobacter lwoffii
2.6.1.76 homologs of dat detected by PCR amplification Acinetobacter genospecies
2.6.1.76 homologs of dat detected by PCR amplification Acinetobacter haemolyticus
2.6.1.76 homologs of dat detected by PCR amplification Acinetobacter junii
2.6.1.76 homologs of dat detected by PCR amplification Acinetobacter radioresistens
2.6.1.76 identification and analysis of the dat gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase with Escherichia coli transformants Acinetobacter baumannii
4.1.1.86 gene dat, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain HB101 Acinetobacter baumannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.76 1.46
-
 2-oxoglutaric acid
-
 Acinetobacter baumannii
2.6.1.76 4.3
-
 L-2,4-diaminobutyric acid
-
 Acinetobacter baumannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.76 45000
-
 4 * 45000, SDS-PAGE Acinetobacter baumannii
2.6.1.76 47420
-
 deduced from nucleotide sequence Acinetobacter baumannii
2.6.1.76 188000
-
 native enzyme, gel filtration Acinetobacter baumannii
4.1.1.86 47423
-
 x * 47423, DNA sequence calculation Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate Acinetobacter baumannii
-
 propane-1,3-diamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.76 Acinetobacter baumannii
-
-
-
2.6.1.76 Acinetobacter calcoaceticus
-
-
-
2.6.1.76 Acinetobacter genospecies
-
 3 ATCC 19004
-
2.6.1.76 Acinetobacter haemolyticus
-
 ATCC 17906
-
2.6.1.76 Acinetobacter johnsonii
-
 ATCC 17909
-
2.6.1.76 Acinetobacter junii
-
 ATCC 17908
-
2.6.1.76 Acinetobacter lwoffii
-
 ATCC 15309
-
2.6.1.76 Acinetobacter radioresistens
-
 IAM13186
-
2.6.1.76 Acinetobacter radioresistens IAM13186
-
 IAM13186
-
4.1.1.86 Acinetobacter baumannii P56744 strain ATCC 19606, gene dat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.76 recombinant enzyme, overexpressed in Escherichia coli Acinetobacter baumannii
4.1.1.86 recombinant enzyme from Escherichia coli strain HB101 to homogeneity by ammonium sulfate fractionation, ion exchange chromatography, gel filtration, and hydroxylapatite chromatography Acinetobacter baumannii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.76 L-2,4-diaminobutanoate + 2-oxoglutarate = L-aspartate 4-semialdehyde + L-glutamate Involved in the formation of 1,3-diaminopropane in Haemophilus influenzae and Acinetobacter baumannii. A product of the ddc gene that also encodes L-2,4-diaminobutyrate decarboxylase in Acinetobacter baumannii. Differs from EC 2.6.1.46 diaminobutyrate-pyruvate transaminase, which has pyruvate as the amino-group acceptor Acinetobacter baumannii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.86 additional information
-
 DABA AT activity compared to other Acinetobacter strains Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.76 beta-alanine + 2-oxoglutaric acid
-
 Acinetobacter baumannii ?
-
 ?
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter calcoaceticus L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter johnsonii L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter lwoffii L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter baumannii L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter genospecies L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter haemolyticus L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter junii L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter radioresistens L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 Acinetobacter radioresistens IAM13186 L-glutamic acid + L-aspartic beta-semialdehyde
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter calcoaceticus L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter johnsonii L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter lwoffii L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter baumannii L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter genospecies L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter haemolyticus L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter junii L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter radioresistens L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-glutamic acid + L-aspartic beta-semialdehyde
-
 Acinetobacter radioresistens IAM13186 L-2,4-diaminobutyric acid + 2-oxoglutaric acid
-
 r
2.6.1.76 L-lysine + oxaloacetic acid
-
 Acinetobacter baumannii ?
-
 ?
2.6.1.76 L-ornithine + pyruvic acid
-
 Acinetobacter baumannii ?
-
 ?
4.1.1.86 4-aminobutanoate
-
 Acinetobacter baumannii propylamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate
-
 Acinetobacter baumannii propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate + 2-oxoglutarate the enzyme is highly specific for 2-oxoglutarate Acinetobacter baumannii L-aspartic beta-semialdehyde + L-glutamic acid
-
 r
4.1.1.86 L-lysine
-
 Acinetobacter baumannii 1,5-diaminopentane + CO2
-
 ?
4.1.1.86 L-ornithine low activity Acinetobacter baumannii 1,4-diaminobutane + CO2
-
 ?
4.1.1.86 additional information substrate specificity of the purified recombinant enzyme, L-2,3-diaminopropionic acid is a poor substrate Acinetobacter baumannii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.76 tetramer 4 * 45000, SDS-PAGE Acinetobacter baumannii
4.1.1.86 ? x * 47423, DNA sequence calculation Acinetobacter baumannii

Synonyms

EC Number Synonyms Comment Organism
4.1.1.86 DABA AT
-
 Acinetobacter baumannii
4.1.1.86 L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase
-
 Acinetobacter baumannii
4.1.1.86 More the enzyme belongs to the subgroup II of the aminotransferases Acinetobacter baumannii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.86 37
-
 assay at Acinetobacter baumannii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.76 8.25 8.75
-
 Acinetobacter baumannii
4.1.1.86 8.5
-
 assay at Acinetobacter baumannii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.86 pyridoxal 5'-phosphate
-
 Acinetobacter baumannii