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Literature summary extracted from

  • Srivenugopal, K.S.; Adiga, P.R.
    Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings (1980), Biochem. J., 190, 461-464.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.44 1,3-diaminopropane
-
 Lathyrus sativus
2.5.1.44 cadaverine
-
 Lathyrus sativus
2.5.1.44 N-ethylmaleimide inhibition suggests, that the enzyme is of a thiol nature Lathyrus sativus
2.5.1.44 spermidine
-
 Lathyrus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.44 3
-
 putrescine
-
 Lathyrus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.44 75000
-
 gel filtration Lathyrus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.44 2 putrescine Lathyrus sativus as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine Santalum album as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 sym-homospermidine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.44 Lathyrus sativus
-
 grass pea
-
2.5.1.44 Santalum album
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.44 partial, 100fold Lathyrus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.44 leaf
-
 Santalum album
-
2.5.1.44 seedling
-
 Lathyrus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.44 0.00025
-
-
 Lathyrus sativus
2.5.1.44 0.0038
-
-
 Santalum album

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.44 2 putrescine as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 Lathyrus sativus sym-homospermidine + NH3
-
 ?
2.5.1.44 2 putrescine as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 Santalum album sym-homospermidine + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.44 37
-
 reaction linear up to 60 min and is proportional to the amount of enzyme protein (0.1-0.5 mg) Lathyrus sativus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.44 4
-
 stable for at least 2 weeks in the presence of 1 mM NAD+ Lathyrus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.44 8.4
-
 in Tris/HCl buffer Lathyrus sativus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.44 NAD+
-
 Santalum album
2.5.1.44 NAD+ absolute requirement Lathyrus sativus