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Literature summary extracted from

  • Pompeo, F.; Bourne, Y.; Van Heijenoort, J.; Fassy, F.; Mengin-Lecreulx, D.
    Dissection of the bifunctional Escherichia coli N-acetylglucosamine-1-phosphate uridyltransferase enzyme into autonomously functional domains and evidence that trimerization is absolutely required for glucosamine-1-phosphate acetyltransferase activity and cell growth (2001), J. Biol. Chem., 276, 3833-3839.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.157
-
 Escherichia coli
2.7.7.23 truncated and full length enzyme overexpressed in Escherichia coli, His-tag inserted for purification Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.157
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.23 DELTA1-130 deletion of N-terminus, very low activity Escherichia coli
2.7.7.23 DELTA1-182 deletion of N-terminus, very low activity Escherichia coli
2.7.7.23 DELTA1-227 deletion of N-terminus, very low activity Escherichia coli
2.7.7.23 DELTA1-233 deletion of N-terminus, very low activity Escherichia coli
2.7.7.23 DELTA1-250 deletion of N-terminus Escherichia coli
2.7.7.23 DELTA1-26 deletion of N-terminus Escherichia coli
2.7.7.23 DELTA1-78 deletion of N-terminus, very low activity Escherichia coli
2.7.7.23 DELTA227-456 deletion of C-terminus Escherichia coli
2.7.7.23 DELTA250-456 deletion of C-terminus, low activity Escherichia coli
2.7.7.23 DELTA331-456 deletion of C-terminus, roughly 50% of activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.157 24700
-
 1 * 37100 + 1 * 24700, uridyltransferase and acetyltransferase in separate domains, SDS-PAGE Escherichia coli
2.3.1.157 37100
-
 1 * 37100 + 1 * 24700, uridyltransferase and acetyltransferase in separate domains, SDS-PAGE Escherichia coli
2.3.1.157 50100
-
-
 Escherichia coli
2.7.7.23 24700
-
 SDS-PAGE, enzyme variant del233 Escherichia coli
2.7.7.23 25600
-
 SDS-PAGE, enzyme variant del227 Escherichia coli
2.7.7.23 28600
-
 SDS-PAGE, enzyme variant Tr250 Escherichia coli
2.7.7.23 36200
-
 SDS-PAGE, enzyme variant del130 Escherichia coli
2.7.7.23 37100
-
 SDS-PAGE, Tr331 Escherichia coli
2.7.7.23 41800
-
 SDS-PAGE, enzyme variant del78 Escherichia coli
2.7.7.23 50100
-
 SDS-PAGE, calculated from DNA sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 D-glucosamine 1-phosphate + acetyl-CoA Escherichia coli
-
 N-acetyl-D-glucosamine 1-phosphate + CoA
-
 ?
2.3.1.157 D-glucosamine 1-phosphate + acetyl-CoA Escherichia coli JM83
-
 N-acetyl-D-glucosamine 1-phosphate + CoA
-
 ?
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Escherichia coli amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine Escherichia coli JM83 amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Escherichia coli
-
 JM83
-
2.3.1.157 Escherichia coli JM83
-
 JM83
-
2.7.7.23 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.157
-
 Escherichia coli
2.7.7.23 full length and truncated enzymes using His-tag Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.157 0.004
-
 mutant JM83(pFP3-Tr331), acetyltransferase activity Escherichia coli
2.3.1.157 0.014
-
 mutant JM83(pTrcHis30), uridyltransferase activity Escherichia coli
2.3.1.157 0.11
-
 mutant JM83(pTrcHis30), acetyltransferase activity Escherichia coli
2.3.1.157 0.12
-
 mutant JM83(pFP3-Tr331), uridyltransferase activity Escherichia coli
2.3.1.157 2.85
-
 mutant JM83(pFP3-Tr331) + IPTG, uridyltransferase activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 D-glucosamine 1-phosphate + acetyl-CoA
-
 Escherichia coli N-acetyl-D-glucosamine 1-phosphate + CoA
-
 ?
2.3.1.157 D-glucosamine 1-phosphate + acetyl-CoA
-
 Escherichia coli JM83 N-acetyl-D-glucosamine 1-phosphate + CoA
-
 ?
2.3.1.157 N-acetyl-D-glucosamine 1-phosphate + UTP glmU gene product, bifunctional enzyme catalyzing 2 subsequent steps in the pathway for UDP-GlcNAc synthesis Escherichia coli UDP-N-acetylglucosamine + ?
-
 r
2.3.1.157 N-acetyl-D-glucosamine 1-phosphate + UTP glmU gene product, bifunctional enzyme catalyzing 2 subsequent steps in the pathway for UDP-GlcNAc synthesis Escherichia coli JM83 UDP-N-acetylglucosamine + ?
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
 Escherichia coli UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Escherichia coli UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine
-
 Escherichia coli JM83 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Escherichia coli JM83 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.157 trimer 1 * 37100 + 1 * 24700, uridyltransferase and acetyltransferase in separate domains, SDS-PAGE Escherichia coli
2.7.7.23 trimer 3 * 50100, SDS-PAGE, gel filtration, all truncated forms form trimers except Tr250 (monomer) Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.157 N-acetylglucosamine-1-phosphate uridyltransferase
-
 Escherichia coli
2.7.7.23 More bifunctional enzyme with activity of: glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.23 0.1
-
 N-acetyl-D-glucosamine 1-phosphate purified del227 enzyme, pH 7.4 Escherichia coli
2.7.7.23 0.2
-
 N-acetyl-D-glucosamine 1-phosphate purified del233 enzyme, pH 7.4 Escherichia coli
2.7.7.23 0.25
-
 N-acetyl-D-glucosamine 1-phosphate purified del78 enzyme, pH 7.4 Escherichia coli
2.7.7.23 0.35
-
 N-acetyl-D-glucosamine 1-phosphate purified del130 enzyme, pH 7.4 Escherichia coli
2.7.7.23 8
-
 N-acetyl-D-glucosamine 1-phosphate purified Tr250 enzyme, pH 7.4 Escherichia coli
2.7.7.23 140
-
 N-acetyl-D-glucosamine 1-phosphate purified Tr331 enzyme, pH 7.4 Escherichia coli
2.7.7.23 330
-
 N-acetyl-D-glucosamine 1-phosphate purified full length enzyme, pH 7.4 Escherichia coli