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Literature summary extracted from

  • Miyazawa, S.; Furuta, S.; Osumi, T.; Hashimoto, T.; Ui, N.
    Properties of peroxisomal 3-ketoacyl-CoA thiolase from rat liver (1981), J. Biochem., 90, 511-519.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.155 cold lability Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.16 additional information
-
 additional information overview: Km of peroxisomal and mitochondrial enzyme with various substrates Rattus norvegicus
2.3.1.155 0.0019
-
 3-ketohexadecanoyl-CoA
-
 Rattus norvegicus
2.3.1.155 0.003
-
 3-oxododecanoyl-CoA
-
 Rattus norvegicus
2.3.1.155 0.008
-
 3-ketodecanoyl-CoA
-
 Rattus norvegicus
2.3.1.155 0.0086
-
 3-ketoocatanoyl-CoA
-
 Rattus norvegicus
2.3.1.155 0.0106
-
 3-ketohexanoyl-CoA
-
 Rattus norvegicus
2.3.1.155 0.014
-
 acetoacetyl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.16 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.1.16 peroxisome
-
 Rattus norvegicus 5777
-
2.3.1.155 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.16 40000
-
 2 * 40000, peroxisomal enzyme, SDS-PAGE Rattus norvegicus
2.3.1.16 41000
-
 2 * 41000, mitochondrial enzyme, SDS-PAGE Rattus norvegicus
2.3.1.16 41000
-
 mitochondrial enzyme, 4 * 41000 Rattus norvegicus
2.3.1.16 89000
-
 peroxisomal enzyme, sedimentation equilibrium Rattus norvegicus
2.3.1.16 154000
-
 mitochondrial enzyme, sedimentation equilibrium Rattus norvegicus
2.3.1.155 38000
-
 gel filtration conducted at 6°C Rattus norvegicus
2.3.1.155 38500
-
 4 * 38500 Rattus norvegicus
2.3.1.155 44500
-
 2 * 44500, SDS-PAGE Rattus norvegicus
2.3.1.155 76000
-
 gel filtration conducted at 28°C Rattus norvegicus
2.3.1.155 78000
-
 peroxisomal bifunctional protein, gel filtration Rattus norvegicus
2.3.1.155 89000
-
 peroxisomal 3-ketoacyl-CoA thiolase, equilibrium centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.155 myristoyl-CoA + acetyl-CoA Rattus norvegicus
-
 3-oxopalmitoyl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.16 Rattus norvegicus
-
-
-
2.3.1.155 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.155
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.16 acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA ping-pong mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.16 liver
-
 Rattus norvegicus
-
2.3.1.155 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Rattus norvegicus acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxodecanoyl-CoA
-
 Rattus norvegicus acetyl-CoA + octanoyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxododecanoyl-CoA
-
 Rattus norvegicus acetyl-CoA + decanoyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxohexadecanoyl-CoA
-
 Rattus norvegicus acetyl-CoA + tetradecanoyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxohexanoyl-CoA
-
 Rattus norvegicus acetyl-CoA + butanoyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxooctanoyl-CoA
-
 Rattus norvegicus acetyl-CoA + hexanoyl-CoA
-
 ?
2.3.1.155 3-ketohexadecanoylCoA + H2O
-
 Rattus norvegicus acetyl-CoA + tetradecanoyl-CoA
-
 r
2.3.1.155 3-oxodecanoyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + octanoyl-CoA
-
 ?
2.3.1.155 3-oxododecanoyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + decanoyl-CoA
-
 ?
2.3.1.155 3-oxohexanoyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + butyryl-CoA
-
 ?
2.3.1.155 3-oxooctanoyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + hexanoyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 myristoyl-CoA + acetyl-CoA
-
 Rattus norvegicus 3-oxopalmitoyl-CoA + CoA
-
 ?
2.3.1.155 tetradecanoyl-CoA + acetyl-CoA myristoyl-CoA Rattus norvegicus 3-ketohexadecanoyl-CoA + CoA 3-oxopalmitoyl-CoA r

Subunits

EC Number Subunits Comment Organism
2.3.1.16 dimer 2 * 40000, peroxisomal enzyme, SDS-PAGE Rattus norvegicus
2.3.1.16 dimer 2 * 41000, mitochondrial enzyme, SDS-PAGE Rattus norvegicus
2.3.1.16 tetramer mitochondrial enzyme, 4 * 41000 Rattus norvegicus
2.3.1.155 dimer 2 * 44500, SDS-PAGE Rattus norvegicus
2.3.1.155 tetramer 4 * 38500 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.155 peroxisomal 3-ketoacyl-CoA thiolase
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.16 additional information
-
 enzyme exhibits reversible cold-lability: loss of acitvity at 4°C in the presence of NaCl, half-maximal inactivation in ice in 4 min Rattus norvegicus