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Literature summary extracted from

  • Kurihara, T.; Ueda, M.; Tanaka, A.
    Peroxisomal acetoacetyl-CoA thiolase and 3-ketoacyl-CoA thiolase from an n-alkane-utilizing yeast, Candida tropicalis: purification and characterization (1989), J. Biochem., 106, 474-478.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.025
-
 acetoacetyl-CoA
-
 Candida tropicalis
2.3.1.16 0.0088
-
 3-oxooctanoyl-CoA
-
 Candida tropicalis
2.3.1.16 0.0093
-
 3-oxododecanoyl-CoA
-
 Candida tropicalis
2.3.1.16 0.01
-
 acetoacetyl-CoA 3-oxodecanoyl-CoA Candida tropicalis
2.3.1.16 0.028
-
 3-oxohexanoyl-CoA
-
 Candida tropicalis
2.3.1.16 0.059
-
 3-oxopentanoyl-CoA
-
 Candida tropicalis
2.3.1.16 0.093
-
 acetoacetyl-CoA
-
 Candida tropicalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.9 peroxisome
-
 Candida tropicalis 5777
-
2.3.1.16 peroxisome
-
 Candida tropicalis 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 41000
-
 6 * 41000, SDS-PAGE Candida tropicalis
2.3.1.16 43000
-
 2 * 43000, SDS-PAGE Candida tropicalis
2.3.1.16 98000
-
 gel filtration Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Candida tropicalis
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Candida tropicalis
-
-
-
2.3.1.16 Candida tropicalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 DEAE-Sepharose, butyl-Toyopearl, Cellulofine Candida tropicalis
2.3.1.16
-
 Candida tropicalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 29
-
-
 Candida tropicalis
2.3.1.16 42
-
-
 Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Candida tropicalis acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Candida tropicalis acetyl-CoA + acetyl-CoA
-
 r
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Candida tropicalis acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxododecanoyl-CoA
-
 Candida tropicalis acetyl-CoA + decanoyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxohexanoyl-CoA
-
 Candida tropicalis acetyl-CoA + butanoyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxooctanoyl-CoA
-
 Candida tropicalis acetyl-CoA + hexanoyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxopentanoyl-CoA
-
 Candida tropicalis acetyl-CoA + propanoyl-CoA
-
 ?
2.3.1.16 CoA + acetoacetyl-CoA
-
 Candida tropicalis acetyl-CoA + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.9 hexamer 6 * 41000, SDS-PAGE Candida tropicalis
2.3.1.16 dimer 2 * 43000, SDS-PAGE Candida tropicalis