BRENDA - Enzyme Database

Identification of the gene and characterization of the activity of the trans-aconitate methyltransferase in Saccharomyces cerevisiae

Cai, H.; Dumlao, D.; Katz, J.E.; Clarke, S.; Biochemistry 40, 13699-13709 (2001)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.144
0.52
-
trans-aconitate
-
Escherichia coli
2.1.1.144
10.4
-
(2R,3S)-isocitrate
-
Escherichia coli
2.1.1.144
23.1
-
DL-isocitrate
-
Escherichia coli
2.1.1.144
36
-
cis-aconitate
-
Escherichia coli
2.1.1.145
0.66
-
trans-aconitate
-
Saccharomyces cerevisiae
2.1.1.145
44
-
Itaconate
-
Saccharomyces cerevisiae
2.1.1.145
74
-
cis-aconitate
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.144
cytosol
-
Escherichia coli
5829
-
2.1.1.145
cytosol
-
Saccharomyces cerevisiae
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
Escherichia coli
-
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
?
2.1.1.145
S-adenosyl-L-methionine + trans-aconitate
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + (E)-2-(methoxycarbonylmethyl)butenedioate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.144
Escherichia coli
-
-
-
2.1.1.145
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.144
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + (2R,3S)-isocitrate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + cis-aconitate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + citrate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + DL-isocitrate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
2.1.1.145
S-adenosyl-L-methionine + cis-aconitate
-
288663
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + (Z)-2-(methoxycarbonylmethyl)butenedioate
-
-
-
?
2.1.1.145
S-adenosyl-L-methionine + itaconate
-
288663
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.145
S-adenosyl-L-methionine + trans-aconitate
-
288663
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + (E)-2-(methoxycarbonylmethyl)butenedioate
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.144
0.52
-
trans-aconitate
-
Escherichia coli
2.1.1.144
10.4
-
(2R,3S)-isocitrate
-
Escherichia coli
2.1.1.144
23.1
-
DL-isocitrate
-
Escherichia coli
2.1.1.144
36
-
cis-aconitate
-
Escherichia coli
2.1.1.145
0.66
-
trans-aconitate
-
Saccharomyces cerevisiae
2.1.1.145
44
-
Itaconate
-
Saccharomyces cerevisiae
2.1.1.145
74
-
cis-aconitate
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.144
cytosol
-
Escherichia coli
5829
-
2.1.1.145
cytosol
-
Saccharomyces cerevisiae
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
Escherichia coli
-
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
?
2.1.1.145
S-adenosyl-L-methionine + trans-aconitate
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + (E)-2-(methoxycarbonylmethyl)butenedioate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.144
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + (2R,3S)-isocitrate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + cis-aconitate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + citrate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + DL-isocitrate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
-
288663
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
2.1.1.145
S-adenosyl-L-methionine + cis-aconitate
-
288663
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + (Z)-2-(methoxycarbonylmethyl)butenedioate
-
-
-
?
2.1.1.145
S-adenosyl-L-methionine + itaconate
-
288663
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.145
S-adenosyl-L-methionine + trans-aconitate
-
288663
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + (E)-2-(methoxycarbonylmethyl)butenedioate
-
-
-
?