BRENDA - Enzyme Database

A novel methyltransferase catalyzes the methyl esterification of trans-aconitate in Escherichia coli

Cai, H.; Clarke, S.; J. Biol. Chem. 274, 13470-13479 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.144
expression in a Escherichia coli BL21
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.144
0.32
-
trans-aconitate
-
Escherichia coli
2.1.1.144
5
-
DL-isocitrate
-
Escherichia coli
2.1.1.144
7.1
-
citrate
-
Escherichia coli
2.1.1.144
9.1
-
(2R,3S)-isocitrate
-
Escherichia coli
2.1.1.144
33
-
cis-aconitate
-
Escherichia coli
2.1.1.144
45
-
citrate
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.144
cytosol
-
Escherichia coli
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
Escherichia coli
-
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.144
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.144
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + (2R,3S)-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + cis-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + citrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + DL-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.144
expression in a Escherichia coli BL21
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.144
0.32
-
trans-aconitate
-
Escherichia coli
2.1.1.144
5
-
DL-isocitrate
-
Escherichia coli
2.1.1.144
7.1
-
citrate
-
Escherichia coli
2.1.1.144
9.1
-
(2R,3S)-isocitrate
-
Escherichia coli
2.1.1.144
33
-
cis-aconitate
-
Escherichia coli
2.1.1.144
45
-
citrate
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.144
cytosol
-
Escherichia coli
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
Escherichia coli
-
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.144
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.144
S-adenosyl-L-methionine + (2R,3S)-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + cis-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + citrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + DL-isocitrate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.144
S-adenosyl-L-methionine + trans-aconitate
-
288662
Escherichia coli
S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate
-
-
-
?