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Literature summary extracted from

  • Wanek, W.; Richter, A.
    Purification and characterization of myo-inositol 6-O-methyltransferase from Vigna umbellata Ohwi et Ohashi (1995), Planta, 197, 427-434.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.129 dithiothreitol 1-20 mM, slight activation Vigna umbellata

General Stability

EC Number General Stability Organism
2.1.1.129 activity is lost within a week, when the enzyme is frozen or kept at 4°C without glycerol Vigna umbellata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.129 1L-1,3,4/2,5-cyclohexanepentol i.e. L-viburnitol Vigna umbellata
2.1.1.129 2,2'-Anhydro-2-C-hydroxymethyl-myo-inositol
-
 Vigna umbellata
2.1.1.129 2,4,6/3,5-pentahydroxy-cyclohexanone i.e. myo-inosose-2 Vigna umbellata
2.1.1.129 Co2+ 5 mM Vigna umbellata
2.1.1.129 Cu2+ 5 mM Vigna umbellata
2.1.1.129 D,L-2,3,4,6/5-pentahydroxy-cyclohexanone i.e. myo-inosose-4 Vigna umbellata
2.1.1.129 EDTA not EGTA Vigna umbellata
2.1.1.129 Fe2+ 5 mM Vigna umbellata
2.1.1.129 Mn2+ 5 mM Vigna umbellata
2.1.1.129 Ni2+ 5 mM Vigna umbellata
2.1.1.129 p-chloromercuribenzenesulfonic acid 0.02 mM Vigna umbellata
2.1.1.129 S-adenosyl-L-homocysteine most potent inhibitor Vigna umbellata
2.1.1.129 Zn2+ 5 mM Vigna umbellata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.129 0.063
-
 S-adenosyl-L-methionine with myo-inositol as cosubstrate Vigna umbellata
2.1.1.129 0.3
-
 D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
-
 Vigna umbellata
2.1.1.129 0.67
-
 2,4,6/3,5-pentahydroxy-cyclohexanone
-
 Vigna umbellata
2.1.1.129 2.92
-
 myo-inositol
-
 Vigna umbellata
2.1.1.129 4.47
-
 1-L-1,3,4/2,5-cyclohexanepentol
-
 Vigna umbellata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.129 K+ 5 mM, slight stimulation Vigna umbellata
2.1.1.129 Na+ 5 mM, slight stimulation Vigna umbellata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.129 40300
-
 2 * or 3 * 40300, SDS-PAGE Vigna umbellata
2.1.1.129 106200
-
 gel filtration Vigna umbellata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.129 S-adenosyl-L-methionine + myo-inositol Vigna umbellata the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.129 Vigna umbellata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.129
-
 Vigna umbellata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.129 stem
-
 Vigna umbellata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.129 additional information
-
-
 Vigna umbellata

Storage Stability

EC Number Storage Stability Organism
2.1.1.129 -30°C, 25 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol, 2 mM MgCl2, 0.25 mM ammonium sulfate, 50% glycerol, half-life: 300 d Vigna umbellata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.129 S-adenosyl-L-methionine + 1L-1,3,4/2,5-cyclohexanepentol i.e. L-viburnitol Vigna umbellata ?
-
 ?
2.1.1.129 S-adenosyl-L-methionine + 2,4,6/3,5-pentahydroxy-cyclohexanone i.e. myo-inosose-2 Vigna umbellata ?
-
 ?
2.1.1.129 S-adenosyl-L-methionine + D,L-2,3,4,6/5-pentahydroxy-cyclohexanone i.e. myo-inosose-4 Vigna umbellata ?
-
 ?
2.1.1.129 S-adenosyl-L-methionine + myo-inositol
-
 Vigna umbellata S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stresses ?
2.1.1.129 S-adenosyl-L-methionine + myo-inositol the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress Vigna umbellata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.129 ? 2 * or 3 * 40300, SDS-PAGE Vigna umbellata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.129 50
-
-
 Vigna umbellata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.129 30 55 30°C: about 40% of maximal activity, 55°C: about 30% of maximal activity Vigna umbellata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.129 45
-
 30 min, stable up to Vigna umbellata
2.1.1.129 50
-
 30 min, about 40% loss of activity Vigna umbellata
2.1.1.129 60
-
 30 min, complete loss of activity Vigna umbellata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.129 7.8
-
-
 Vigna umbellata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.129 7 8.8 about 35% of maximal activity at pH 7.0 and pH 8.8 Vigna umbellata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.129 5.5
-
 3 h, at room temperature, about 45% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata
2.1.1.129 6.5
-
 3 h, at room temperature, about 10% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata
2.1.1.129 7 7.6 3 h, at room temperature, maximal stability Vigna umbellata
2.1.1.129 8
-
 3 h, at room temperature, about 30% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata
2.1.1.129 9
-
 3 h, at room temperature, about 70% loss of activity compared to maximal stability at pH 7.0-7.6 Vigna umbellata