Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suter, M.; von Ballmoos, P.; Kopriva, S.; Op den Camp, R.; Schaller, J.; Kuhlemeier, C.; Schurmann, P.; Brunold, C.
    Adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes (2000), J. Biol. Chem., 275, 930-936.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.9
-
 Lemna minor

General Stability

EC Number General Stability Organism
1.8.4.9 enzyme unstable during purification and storage, addition of 5 mM 5'-adenosine monophosphate stabilizes activity Lemna minor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.4.9 5'-adenylylsulfate at 0.12 mM Lemna minor
1.8.4.9 adenosine-5'-phosphate competitive inhibition, Ki of 0.34 mM with 10 mM glutathione Lemna minor
1.8.4.9 glutathione at 60 mM Lemna minor
2.8.2.B1 5'-AMP
-
 Lemna minor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.9 0.0065
-
 5'-adenylylsulfate with 10 mM glutathione as reductant Lemna minor
1.8.4.9 0.0122
-
 5'-adenylylsulfate with 40 mM glutathione as reductant Lemna minor
1.8.4.9 16.8
-
 glutathione with 0.04 mM adenylylsulfate Lemna minor
2.8.2.B1 additional information
-
 additional information
-
 Lemna minor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.9 chloroplast sequence contains chloroplast transit peptide Lemna minor 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.9 43000
-
 SDS-PAGE Lemna minor
1.8.4.9 43640
-
 calculated from cDNA sequence Lemna minor
1.8.4.9 91300
-
 estimation by gel filtration with Superose 12 Lemna minor
2.8.2.B1 43638
-
 1 * 43638, calculated from sequence Lemna minor
2.8.2.B1 91300
-
 gel filtration Lemna minor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.99.2 adenylyl sulfate + reduced acceptor Lemna minor key enzyme of assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.9 Lemna minor
-
-
-
1.8.99.2 Lemna minor
-
-
-
2.8.2.B1 Lemna minor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.9 purification to homogeneity Lemna minor
2.8.2.B1 enzyme is purified from Lemna as well as recombinant enzyme Lemna minor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.9 0.02 0.025 crude extract, plants treated with 10 mM NH4Cl Lemna minor
2.8.2.B1 15
-
-
 Lemna minor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.9 3'-phosphoadenosine-5'-phosphosulfate + glutathione no activity with 3'-phosphoadenosine-5'-phosphosulfate Lemna minor adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
 ?
1.8.4.9 5'-adenylylsulfate + dithioerythritol
-
 Lemna minor adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
-
 ?
1.8.4.9 5'-adenylylsulfate + glutathione
-
 Lemna minor adenosine monophosphate + sulfite + oxidized glutathione
-
 ?
1.8.99.2 adenylyl sulfate + GSH
-
 Lemna minor AMP + sulfite + GSSG
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor key enzyme of assimilatory sulfate reduction Lemna minor AMP + sulfite + acceptor
-
 ?
1.8.99.2 additional information adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes Lemna minor ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + glutathione
-
 Lemna minor SO32- + ? enzyme form almost exclusively SO32- ?

Subunits

EC Number Subunits Comment Organism
1.8.4.9 dimer alpha2, 2 * 43000, comparison of native mass after gel filtration with denatured after SDS-PAGE Lemna minor
2.8.2.B1 monomer 1 * 43638, calculated from sequence Lemna minor

Synonyms

EC Number Synonyms Comment Organism
2.8.2.B1 adenosine 5'-phosphosulfate reductase
-
 Lemna minor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.9 56
-
 5'-adenylylsulfate
-
 Lemna minor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.B1 0.24
-
 5'-AMP with 40 mM GSH Lemna minor
2.8.2.B1 0.34
-
 5'-AMP with 10 mM GSH Lemna minor