Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gutierrez-Marcos, J.F.; Roberts, M.A.; Campbell, E.I.; Wray, J.L.
    Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and "APS reductase" activity (1996), Proc. Natl. Acad. Sci. USA, 93, 13377-13382.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.9
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.9 chloroplast sequence contains chloroplast transit peptide Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.9 43000
-
 mature protein without chloroplast transit peptide, calculated from cDNA sequence Arabidopsis thaliana
1.8.4.9 50450
-
 clone Prh-43, calculated from cDNA sequence Arabidopsis thaliana
1.8.4.9 50500
-
 clone Prh-26, calculated from cDNA sequence Arabidopsis thaliana
1.8.4.9 51300
-
 clone Prh-19, calculated from cDNA sequence Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.9 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.9 purification of recombinant 6xHis-tagged protein with Nickel-affinity chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.9 0.000002
-
 with 3'-phosphoadenosine 5'-phosphosulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli Arabidopsis thaliana
1.8.4.9 0.00059
-
 with 5'-adenylylsulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli Arabidopsis thaliana
1.8.4.9 0.013
-
 after purification of His-tagged protein with Nickel-affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.9 3'-phosphoadenosine-5'-phosphosulfate + glutathione
-
 Arabidopsis thaliana adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
 ?
1.8.4.9 5'-adenylylsulfate + glutathione
-
 Arabidopsis thaliana adenosine monophosphate + sulfite + oxidized glutathione
-
 ?