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Literature summary extracted from
- Sulfate reduction in higher plants: Molecular evidence for a novel 5'-adenylylsulfate reductase (1996), Proc. Natl. Acad. Sci. USA, 93, 13383-13388.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.9 | (NH4)2SO4 | less effective than Na2SO4 | Arabidopsis thaliana |  |
| 1.8.4.9 | DTT | DTT can replace glutathione as reductant, 5 mM DTT increases activity 40fold | Arabidopsis thaliana | |
| 1.8.4.9 | Na2SO4 | - |
Arabidopsis thaliana | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.9 | - |
Arabidopsis thaliana |
| 1.8.99.2 | - |
Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.9 | adenosine-5'-phosphate | - |
Arabidopsis thaliana | |
| 1.8.99.2 | 2'-AMP | - |
Arabidopsis thaliana | |
| 1.8.99.2 | 3'-phosphoadenosine-5'-phosphate | - |
Arabidopsis thaliana | |
| 1.8.99.2 | 5'-AMP | - |
Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.4.9 | chloroplast | sequence contains chloroplast transit peptide | Arabidopsis thaliana | 9507 | - |
| 1.8.99.2 | additional information | mitochondrial or cytoplasmic localization | Arabidopsis thaliana | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | additional information | stimulation by high ionic strength | Arabidopsis thaliana |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.9 | 45600 | - |
clone APR2, calculated from cDNA sequence | Arabidopsis thaliana |
| 1.8.4.9 | 47300 | - |
clone APR3, calculated from cDNA sequence | Arabidopsis thaliana |
| 1.8.4.9 | 50800 | - |
clone APR1, calculated from cDNA sequence | Arabidopsis thaliana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.9 | Arabidopsis thaliana | - |
- |
- |
| 1.8.99.2 | Arabidopsis thaliana | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.9 | 0.000007 | - |
with 3'-phosphoadenosine 5'-phosphosulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli | Arabidopsis thaliana |
| 1.8.4.9 | 0.00022 | - |
with 5'-adenylylsulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.9 | 3'-phosphoadenosine-5'-phosphosulfate + glutathione | 50fold lower activity than with 5'-adenylylsulfate | Arabidopsis thaliana | adenosine 3',5'-diphosphate + sulfite + oxidized glutathione | - |
? | |
| 1.8.4.9 | 5'-adenylylsulfate + dithiothreitol | - |
Arabidopsis thaliana | adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol | - |
? | |
| 1.8.4.9 | 5'-adenylylsulfate + glutathione | - |
Arabidopsis thaliana | adenosine monophosphate + sulfite + oxidized glutathione | - |
? | |
| 1.8.99.2 | adenylyl sulfate + dithiothreitol | - |
Arabidopsis thaliana | AMP + sulfite + ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.9 | 8.5 | - |
recombinant protein, expressed in Escherichia coli | Arabidopsis thaliana |
| 1.8.99.2 | 8.5 | - |
reaction with adenylyl sulfate and dithiothreitol | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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