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Literature summary extracted from

  • Clark, D.D.; Allen, J.R.; Ensign, S.A.
    Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system (2000), Biochemistry, 39, 1294-1304.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.5 N-ethylmaleimide
-
 Xanthobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.5 6.3
-
 acetoacetate
-
 Xanthobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.5 57000
-
 molecular weight of subunits Xanthobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.5 2-mercaptoethanesulfonate + acetoacetate + NADP+ Xanthobacter sp. involved in epoxide carboxylation pathway in bacteria, reverse reaction more important 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 r
1.8.1.5 2-mercaptoethanesulfonate + acetoacetate + NADP+ Xanthobacter sp. Py2 involved in epoxide carboxylation pathway in bacteria, reverse reaction more important 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.5 Xanthobacter sp.
-
 Py2
-
1.8.1.5 Xanthobacter sp. Py2
-
 Py2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.5 0.0041
-
 rate of decarboxylation without added cofactors Xanthobacter sp.
1.8.1.5 0.274
-
 NADPH formation Xanthobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.5 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 Xanthobacter sp. 2-mercaptoethanesulfonate + acetoacetate + NADP+
-
 r
1.8.1.5 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 Xanthobacter sp. Py2 2-mercaptoethanesulfonate + acetoacetate + NADP+
-
 r
1.8.1.5 2-(2-oxopropylthio)ethanesulfonate + NADPH + H+
-
 Xanthobacter sp. acetone + 2-mercaptoethanesulfonate + NADP+
-
 ir
1.8.1.5 2-(2-oxopropylthio)ethanesulfonate + NADPH + H+
-
 Xanthobacter sp. Py2 acetone + 2-mercaptoethanesulfonate + NADP+
-
 ir
1.8.1.5 2-mercaptoethanesulfonate + acetoacetate + NADP+
-
 Xanthobacter sp. 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 r
1.8.1.5 2-mercaptoethanesulfonate + acetoacetate + NADP+ involved in epoxide carboxylation pathway in bacteria, reverse reaction more important Xanthobacter sp. 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 r
1.8.1.5 2-mercaptoethanesulfonate + acetoacetate + NADP+
-
 Xanthobacter sp. Py2 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 r
1.8.1.5 2-mercaptoethanesulfonate + acetoacetate + NADP+ involved in epoxide carboxylation pathway in bacteria, reverse reaction more important Xanthobacter sp. Py2 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 r
1.8.1.5 3-mercaptopropanesulfonate + acetoacetate + NADP+ very poor cofactor, 1% of activity Xanthobacter sp. 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 ?
1.8.1.5 3-mercaptopropanesulfonate + acetoacetate + NADP+ very poor cofactor, 1% of activity Xanthobacter sp. Py2 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+
-
 ?
1.8.1.5 3-mercaptopropionate + acetoacetate + NADP+ poor cofactor, 11% of activity Xanthobacter sp. 3-(2-oxopropylthio)propionate + CO2 + NADPH + H+
-
 ?
1.8.1.5 acetoacetate + H+ very low rate Xanthobacter sp. acetone + CO2
-
 ?
1.8.1.5 CO2 + acetoacetate exchange of C-14 without added cofactors Xanthobacter sp. acetoacetate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.5 dimer 2 * 57000 Xanthobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.5 0.263
-
 acetoacetate
-
 Xanthobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.5 2-mercaptoethanesulfonic acid coenzyme M Xanthobacter sp.
1.8.1.5 FAD enzyme bound Xanthobacter sp.