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Literature summary extracted from
- Expression, purification, and characterization of Mycobacterium tuberculosis mycothione reductase (1999), Biochemistry, 38, 11827-11833.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.15 | - |
Mycobacterium tuberculosis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.15 | 0.001 | - |
thio-NADPH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis |  |
| 1.8.1.15 | 0.008 | - |
beta-NADPH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.023 | - |
reduced beta-nicotinamide hypoxanthine dinucleotide phosphate | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.043 | - |
NADH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.055 | - |
alpha-NADPH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.073 | - |
mycothione | - |
Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.09 | - |
thio-NADH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.2 | - |
reduced beta-nicotinamide hypoxanthine dinucleotide | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.24 | - |
2-methyl-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.34 | - |
5,8-Dihydroxy-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.51 | - |
des-myo-inositol mycothione | - |
Mycobacterium tuberculosis | |
| 1.8.1.15 | 0.54 | - |
5-hydroxy-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | |
| 1.8.1.15 | 3.3 | - |
DTNB | - |
Mycobacterium tuberculosis | |
| 1.8.1.15 | 4 | - |
2,6-dimethylbenzoquinone | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.15 | 50000 | - |
alpha2, 2 * 50000, SDS-PAGE | Mycobacterium tuberculosis |
| 1.8.1.15 | 100000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.15 | Mycobacterium tuberculosis | P9WHH3 | - |
- |
| 1.8.1.15 | Mycobacterium tuberculosis H37Rv | P9WHH3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.15 | - |
Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.15 | 3.63 | - |
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.15 | 2,6-dimethylbenzoquinone + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | 2,6-dimethylbenzoquinone + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 1.8.1.15 | 2-methyl-1,4-naphthoquinone + alpha-NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | 5,8-dihydroxy-1,4-naphthoquinone + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | 5-hydroxy-1,4-naphthoquinone + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | 5-hydroxy-1,4-naphthoquinone + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 1.8.1.15 | des-myo-inositol mycothione + NADH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | des-myo-inositol mycothione + reduced beta-nicotinamide hypoxanthine dinucleotide | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | des-myo-inositol mycothione + reduced beta-nicotinamide hypoxanthine dinucleotide phosphate | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | des-myo-inositol mycothione + thio-NADH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | des-myo-inositol mycothione + thio-NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | desmyoinositol mycothione + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | desmyoinositol mycothione + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 1.8.1.15 | DTNB + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| 1.8.1.15 | DTNB + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| 1.8.1.15 | mycothione + NADPH | - |
Mycobacterium tuberculosis | mycothiol + NADP+ | - |
? | |
| 1.8.1.15 | mycothione + NADPH | - |
Mycobacterium tuberculosis H37Rv | mycothiol + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.15 | homodimer | alpha2, 2 * 50000, SDS-PAGE | Mycobacterium tuberculosis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.15 | FAD | 1 mol FAD per monomer | Mycobacterium tuberculosis | |
| 1.8.1.15 | NADPH | - |
Mycobacterium tuberculosis | |
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