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Literature summary extracted from

  • Kajie, S.; Anraku, Y.
    Purification of a hexaheme cytochrome c552 from Escherichia coli K 12 and its properties as a nitrite reductase (1986), Eur. J. Biochem., 154, 457-463.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.2 CuCl2 0.01 mM,70% inhibition; 0.1 mM, 69% inhibition Escherichia coli K-12
1.7.2.2 CuSO4 0.01 mM, 68% inhibition Escherichia coli K-12
1.7.2.2 KCN 0.1 mM, 84% inhibition; 1 mM, 100% Escherichia coli K-12
1.7.2.2 KNO3 1 mM, 6% inhibition Escherichia coli K-12
1.7.2.2 NaN3 1 mM, 16% inhibition Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.2 0.11
-
 nitrite
-
 Escherichia coli K-12
1.7.2.2 18
-
 hydroxylamine
-
 Escherichia coli K-12

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.2 additional information no non-heme iron Escherichia coli K-12

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.2 69000
-
 SDS-PAGE Escherichia coli K-12
1.7.2.2 72000
-
 native enzyme, gel filtration Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Escherichia coli K-12
-
 ST249
-
1.7.2.2 Escherichia coli K-12 ST249
-
 ST249
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.2
-
 Escherichia coli K-12

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.2 36
-
 FADH2 Escherichia coli K-12
1.7.2.2 50
-
 FMNH2 Escherichia coli K-12
1.7.2.2 2690
-
 hydroxylamine Escherichia coli K-12
1.7.2.2 2930
-
 nitrite Escherichia coli K-12
1.7.2.2 2930
-
 benzyl viologen Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 hydroxylamine + benzyl viologen reduced
-
 Escherichia coli K-12 ? + benzyl viologen oxidized
-
 ?
1.7.2.2 hydroxylamine + benzyl viologen reduced
-
 Escherichia coli K-12 ST249 ? + benzyl viologen oxidized
-
 ?
1.7.2.2 additional information no nitrate or sulfite reductase activity Escherichia coli K-12 ?
-
 ?
1.7.2.2 additional information not accepted electron donors: NADH, NADPH, ubiquinol-1, menadiol, formate, D-lactate, sn-glycerol-3-phosphate Escherichia coli K-12 ?
-
 ?
1.7.2.2 additional information no nitrate or sulfite reductase activity Escherichia coli K-12 ST249 ?
-
 ?
1.7.2.2 additional information not accepted electron donors: NADH, NADPH, ubiquinol-1, menadiol, formate, D-lactate, sn-glycerol-3-phosphate Escherichia coli K-12 ST249 ?
-
 ?
1.7.2.2 nitrite + benzyl viologen reduced + H+
-
 Escherichia coli K-12 NH4+ + benzyl viologen oxidized + H2O
-
 ?
1.7.2.2 nitrite + benzyl viologen reduced + H+
-
 Escherichia coli K-12 ST249 NH4+ + benzyl viologen oxidized + H2O
-
 ?
1.7.2.2 nitrite + FADH2
-
 Escherichia coli K-12 NH4+ + FAD
-
 ?
1.7.2.2 nitrite + FADH2
-
 Escherichia coli K-12 ST249 NH4+ + FAD
-
 ?
1.7.2.2 nitrite + FMNH2
-
 Escherichia coli K-12 NH4+ + FMN
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.2 monomer 1 * 69000, SDS-PAGE Escherichia coli K-12

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.2.2 10 60 activity increases up to 60 Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.2 7.5
-
-
 Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.2 heme c
-
 Escherichia coli K-12