Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bertrand, J.C.; Bazin, H.; Zacek, M.; Gilewicz, M.; Azoulay, E.
    NADPH-cytochrome c reductase of Candida tropicalis grown on alkane (1979), Eur. J. Biochem., 93, 237-243.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 HgCl2 cells grown on alkane or glycerol, presence of NADPH provides protection against inhibition Candida tropicalis
1.6.2.4 additional information increasing ionic strength, grown on alkane or glycerol Candida tropicalis
1.6.2.4 NADP+
-
 Candida tropicalis
1.6.2.4 p-chloromercuribenzoate cells grown on alkane or glycerol, presence of NADPH provides protection against inhibition Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0043
-
 ferricytochrome c grown on glycerol Candida tropicalis
1.6.2.4 0.00455
-
 ferricytochrome c grown on alkanes Candida tropicalis
1.6.2.4 0.029
-
 NADPH grown on alkanes Candida tropicalis
1.6.2.4 0.033
-
 NADPH grown on glycerol Candida tropicalis
1.6.2.4 0.055
-
 oxidized 2,6-dichlorophenolindophenol grown on glycerol Candida tropicalis
1.6.2.4 0.077
-
 oxidized 2,6-dichlorophenolindophenol grown on alkanes Candida tropicalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 65000 68000 gel filtration, sedimentation equilibrium centrifugation Candida tropicalis
1.6.2.4 67000
-
 1 * 67000, SDS-PAGE, trypsin- or lipase-solubilized Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 additional information Candida tropicalis monooxygenase system only present in cells grown on alkanes ?
-
 ?
1.6.2.4 additional information Candida tropicalis monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids ?
-
 ?
1.6.2.4 NADPH + ferricytochrome P450 Candida tropicalis during hydroxylation of alkanes ferrocytochrome P450 + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Candida tropicalis
-
 grown on alkanes or glycerol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 DEAE-cellulose and Sephadex G-100 column chromatography Candida tropicalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.1 0.3 grown on glycerol, microsomal fraction Candida tropicalis
1.6.2.4 0.75 1.1 grown on alkane, microsomal fraction Candida tropicalis
1.6.2.4 12.5
-
 grown on alkane, electron acceptor: neotetrazolium chloride, purified enzyme Candida tropicalis
1.6.2.4 18.5
-
 grown on alkane, electron acceptor: ferricyanide, purified enzyme Candida tropicalis
1.6.2.4 29
-
 grown on alkane, electron acceptor: menadione, purified enzyme Candida tropicalis
1.6.2.4 50
-
 grown on alkane, electron acceptor: dichlorophenolindophenol, purified enzyme Candida tropicalis
1.6.2.4 70
-
 grown on alkane, electron acceptor: cytochrome c, purified enzyme Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: menadione Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 ferricytochrome c + NADPH + H+
-
 Candida tropicalis ferrocytochrome c + NADP+
-
 r
1.6.2.4 additional information monooxygenase system only present in cells grown on alkanes Candida tropicalis ?
-
 ?
1.6.2.4 additional information monooxygenase system composed of cytochrome P-450, NADPH-cytochrome c reductase, phospholipids Candida tropicalis ?
-
 ?
1.6.2.4 NADPH + ferricytochrome P450 during hydroxylation of alkanes Candida tropicalis ferrocytochrome P450 + NADP+
-
 ?
1.6.2.4 NADPH + H+ oxidized 2,6-dichlorophenolindophenol
-
 Candida tropicalis ferrocytochrome c + reduced 2,6-dichlorophenolindophenol
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.4 monomer 1 * 67000, SDS-PAGE, trypsin- or lipase-solubilized Candida tropicalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 102
-
 ferricytochrome c grown on alkanes Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.8 8 grown on alkane or glycerol Candida tropicalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.2.4 7 8.5 less than 50% of maximal activity above and below, grown on alkane or glycerol Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD ratio FAD : FMN 1 : 1 Candida tropicalis
1.6.2.4 FMN ratio FAD : FMN 1 : 1 Candida tropicalis
1.6.2.4 NADPH
-
 Candida tropicalis