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Literature summary extracted from
- Purification and characterization of the NADPH-cytochrome P-450 (cytochrome c) reductase from higher-plant microsomal fraction (1986), Biochem. J., 235, 365-373.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | additional information | low ionic strength, slight stimulation | Helianthus tuberosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | additional information | high ionic strength | Helianthus tuberosus | |
| 1.6.2.4 | NADP+ | non-competitive inhibition | Helianthus tuberosus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.4 | additional information | - |
additional information | cosubstrate NADH, biphasic kinetic | Helianthus tuberosus | |
| 1.6.2.4 | 0.0182 | - |
ferricytochrome c | determined on the basis of a sequential mechanism | Helianthus tuberosus | |
| 1.6.2.4 | 0.02 | - |
NADPH | - |
Helianthus tuberosus | |
| 1.6.2.4 | 0.0308 | - |
NADPH | determined on the basis of a sequential mechanism | Helianthus tuberosus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.2.4 | microsome | - |
Helianthus tuberosus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | additional information | stimulation by increasing ionic strength | Helianthus tuberosus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | additional information | - |
differences in MW partially due to method of solubilization | Helianthus tuberosus |
| 1.6.2.4 | 82000 | - |
x * 82000, SDS-PAGE | Helianthus tuberosus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.4 | NADPH + ferricytochrome P450 | Helianthus tuberosus | part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 | ferrocytochrome P450 + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | Helianthus tuberosus | - |
L. var. Blanc commun., Jerusalem artichoke | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | anion exchange chromatography and affinity chromatography | Helianthus tuberosus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | reconstitution of monooxygenase system | Helianthus tuberosus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | tuber | - |
Helianthus tuberosus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | 33 | - |
- |
Helianthus tuberosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Helianthus tuberosus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: cytochrome P450 | Helianthus tuberosus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | ferricytochrome c + NADPH + H+ | - |
Helianthus tuberosus | ferrocytochrome c + NADP+ | - |
r | |
| 1.6.2.4 | additional information | hydroxylation of coumarate | Helianthus tuberosus | ? | - |
? | |
| 1.6.2.4 | NADPH + ferricytochrome P450 | part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 | Helianthus tuberosus | ferrocytochrome P450 + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | ? | x * 82000, SDS-PAGE | Helianthus tuberosus |
| 1.6.2.4 | More | differences in subunit weight partially due to method of solubilization | Helianthus tuberosus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | FAD | ratio FAD : FMN 1 : 1 | Helianthus tuberosus | |
| 1.6.2.4 | FMN | ratio FAD : FMN 1 : 1 | Helianthus tuberosus | |
| 1.6.2.4 | FMN | not implicated in plant NADPH-ferricyanide reductase | Helianthus tuberosus | |
| 1.6.2.4 | NADH | low affinity | Helianthus tuberosus | |
| 1.6.2.4 | NADPH | - |
Helianthus tuberosus | |
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