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Literature summary extracted from

  • te Brommelstroet, B.W.; Geerts, W.J.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.
    Purification and properties of 5,10-methylenetetrahydromethanopterin dehydrogenase and 5,10-methylenetetrahydromethanopterin reductase, two coenzyme F420-dependent enzymes, from Methanosarcina barkeri (1991), Biochim. Biophys. Acta, 1079, 293-302.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.1 0.006
-
 5,10-methylenetetrahydromethanopterin
-
 Methanosarcina barkeri
1.5.98.1 0.018
-
 coenzyme F420
-
 Methanosarcina barkeri
1.5.98.2 0.25
-
 N5,N10-methylenetetrahydromethanopterin
-
 Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.1 35000
-
 6 * 35000, SDS-PAGE Methanosarcina barkeri
1.5.98.1 200000
-
 gel filtration Methanosarcina barkeri
1.5.98.1 216000
-
 native PAGE Methanosarcina barkeri
1.5.98.2 38000
-
 6 * 38000, SDS-PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 Methanosarcina barkeri the enzyme is proposed to function in methanol oxidation step to CO2 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.2 N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 Methanosarcina barkeri
-
 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Methanosarcina barkeri
-
-
-
1.5.98.2 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.1
-
 Methanosarcina barkeri
1.5.98.2
-
 Methanosarcina barkeri

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 ternary complex mechanism Methanosarcina barkeri
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.1 670
-
-
 Methanosarcina barkeri
1.5.98.2 31.5
-
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
 Methanosarcina barkeri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 the enzyme is proposed to function in methanol oxidation step to CO2 Methanosarcina barkeri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.2 N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420
-
 Methanosarcina barkeri 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.98.1 hexamer 6 * 35000, SDS-PAGE Methanosarcina barkeri
1.5.98.2 hexamer 6 * 38000, SDS-PAGE Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.1 60 65
-
 Methanosarcina barkeri
1.5.98.2 65
-
-
 Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.1 6 6.7
-
 Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420 required Methanosarcina barkeri