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Literature summary extracted from

  • Short, J.D.; Guo, D.A.; Svoboda, J.A.; Nes, W.D.
    Mechanistic and metabolic studies of sterol 24,25-double bond reduction in Manduca sexta (1996), Arch. Insect Biochem. Physiol., 31, 1-22.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.3.1.72 agriculture inhibition of phytosterol metabolism to cholesterol by interfering with DELTA24-sterol reductase activity presents a unique target site that might be exploited further as a selective, biorational insect-control technology Manduca sexta

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.72 24(R,S),25-epimino-lanosterol iminiolanosterol, IL, potent inhibitor, IC50: 0.002 mM Manduca sexta
1.3.1.72 25-Azacholesterol
-
 Manduca sexta
1.3.1.72 azasteroids mechanism of inhibition Manduca sexta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.72 additional information
-
 additional information kinetic data Manduca sexta
1.3.1.72 0.0026
-
 5alpha-cholesta-5,24-dien-3beta-ol desmosterol Manduca sexta
1.3.1.72 0.018
-
 4,4',14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol lanosterol Manduca sexta
1.3.1.72 0.033
-
 Cycloartenol
-
 Manduca sexta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.72 microsome microsome-bound Manduca sexta
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.72 desmosterol + NADPH Manduca sexta 5alpha-cholesta-5,24-dien-3beta-ol cholesterol + NADP+
-
 ?
1.3.1.72 additional information Manduca sexta enzyme of phytosterol metabolism to cholesterol ?
-
 ?
1.3.1.72 additional information Manduca sexta reduction of 24,25-double bond ?
-
 ?
1.3.1.72 additional information Manduca sexta insects are unable to synthesize sterols de novo and acquire sterols from their diet or from symbionts, sterol 24,25-reductase is an important enzyme of the pathway for the transformation of sitosterol, 24alpha-ethylcholesterol, together with stigmasterol, 24alpha-ethylcholesta-5,22-dien-3beta-ol, the predominant phytosterol in tobacco, to cholesterol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.72 Bombyx mori
-
 silkworm
-
1.3.1.72 Manduca sexta
-
 tobacco hornworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.72
-
 Manduca sexta

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.72 5alpha-cholest-7-en-3beta-ol + NADP+ = 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+ mechanism Manduca sexta
a
1.3.1.72 5alpha-cholest-7-en-3beta-ol + NADP+ = 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+ stereochemistry Bombyx mori
a
1.3.1.72 5alpha-cholest-7-en-3beta-ol + NADP+ = 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+ stereochemistry Manduca sexta
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.72 larva
-
 Manduca sexta
-
1.3.1.72 midgut
-
 Manduca sexta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.72 additional information
-
-
 Manduca sexta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.72 5alpha-cholesta-7,24-dien-3beta-ol + NADPH
-
 Manduca sexta 5alpha-cholesta-7-en-3beta-ol + NADP+ lathosterol ?
1.3.1.72 cycloartenol + NADPH + H+
-
 Manduca sexta cycloartanol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH preferred, best substrate Manduca sexta cholesterol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH 5alpha-cholesta-5,24-dien-3beta-ol Bombyx mori cholesterol + NADP+
-
 ?
1.3.1.72 desmosterol + NADPH 5alpha-cholesta-5,24-dien-3beta-ol Manduca sexta cholesterol + NADP+
-
 ?
1.3.1.72 lanosterol + NADPH 4,4',14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol Manduca sexta 4,4,14alpha-trimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+
-
 ?
1.3.1.72 additional information substrate specificity Manduca sexta ?
-
 ?
1.3.1.72 additional information sterol 24,25-double bond reduction Bombyx mori ?
-
 ?
1.3.1.72 additional information sterol 24,25-double bond reduction Manduca sexta ?
-
 ?
1.3.1.72 additional information substrate specificity studies Manduca sexta ?
-
 ?
1.3.1.72 additional information enzyme of phytosterol metabolism to cholesterol Manduca sexta ?
-
 ?
1.3.1.72 additional information reduction of 24,25-double bond Manduca sexta ?
-
 ?
1.3.1.72 additional information insects are unable to synthesize sterols de novo and acquire sterols from their diet or from symbionts, sterol 24,25-reductase is an important enzyme of the pathway for the transformation of sitosterol, 24alpha-ethylcholesterol, together with stigmasterol, 24alpha-ethylcholesta-5,22-dien-3beta-ol, the predominant phytosterol in tobacco, to cholesterol Manduca sexta ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.72 37
-
 assay at Manduca sexta

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.72 NADPH
-
 Bombyx mori
1.3.1.72 NADPH NADPH-linked Manduca sexta

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.72 0.002
-
 iminiolanosterol, IL, potent inhibitor, IC50: 0.002 mM Manduca sexta 24(R,S),25-epimino-lanosterol