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Literature summary extracted from

  • Baloch, R.I.; Mercer, E.I.
    Inhibition of sterol DELTA8 -> DELTA7-isomerase and DELTA14-reductase by fenpropimorph, tridemorph and fenpropidin in cell-free enzyme systems from Saccharomyces cerevisiae (1987), Phytochemistry, 26, 663-668.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.70 fenpropidin fungicide, very potent inhibitor; IC50: 0.0018 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine Saccharomyces cerevisiae
1.3.1.70 fenpropidin fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine Ustilago maydis
1.3.1.70 fenpropimorph fungicide, very potent inhibitor; IC50: 0.0023 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine Saccharomyces cerevisiae
1.3.1.70 fenpropimorph fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine Ustilago maydis
1.3.1.70 tridemorph 2,6-dimethyl-N-tridecylmorpholine; fungicide; slight inhibitor, IC50: 0.098 mM Saccharomyces cerevisiae
1.3.1.70 tridemorph 2,6-dimethyl-N-tridecylmorpholine; fungicide Ustilago maydis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.70 additional information Saccharomyces cerevisiae essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol ?
-
 ?
1.3.1.70 additional information Saccharomyces cerevisiae NCYC 739 essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.70 Saccharomyces cerevisiae
-
 NCYC 739
-
1.3.1.70 Saccharomyces cerevisiae NCYC 739
-
 NCYC 739
-
1.3.1.70 Ustilago maydis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.70 partial Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.70 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ mechanism Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.70 5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH
-
 Saccharomyces cerevisiae 5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+
-
 ?
1.3.1.70 5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH
-
 Saccharomyces cerevisiae NCYC 739 5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+
-
 ?
1.3.1.70 additional information essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol Saccharomyces cerevisiae ?
-
 ?
1.3.1.70 additional information essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol Saccharomyces cerevisiae NCYC 739 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.70 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.70 6.2
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.70 NADPH
-
 Saccharomyces cerevisiae
1.3.1.70 NADPH
-
 Ustilago maydis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.70 0.0018
-
 IC50: 0.0018 mM Saccharomyces cerevisiae fenpropidin
1.3.1.70 0.0023
-
 IC50: 0.0023 mM Saccharomyces cerevisiae fenpropimorph
1.3.1.70 0.098
-
 slight inhibitor, IC50: 0.098 mM Saccharomyces cerevisiae tridemorph