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Literature summary extracted from

  • Roberti, R.; Bennati, A.M.; Galli, G.; Caruso, D.; Maras, B.; Aisa, C.; Beccari, T.; Della Fazia, M.A.; Servillo, G.
    Cloning and expression of sterol DELTA14-reductase from bovine liver (2002), Eur. J. Biochem., 269, 283-290.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.70 cDNA expression in COS-7 cells Bos taurus
1.3.1.70 RT-PCR cloning Bos taurus
1.3.1.70 sterol DELTA14-reductase mRNA expression in bovine tisssues, high levels in liver and brain Bos taurus
1.3.1.70 TM7SF2 gene corresponds to DELTA14-SR, sterol DELTA14-reductase Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.70 endoplasmic reticulum integral protein of endoplasmic reticulum Homo sapiens 5783
-
1.3.1.70 endoplasmic reticulum integral protein of endoplasmic reticulum Bos taurus 5783
-
1.3.1.70 membrane transmembrane domains Bos taurus 16020
-
1.3.1.70 microsome
-
 Homo sapiens
-
-
1.3.1.70 microsome
-
 Bos taurus
-
-
1.3.1.70 additional information subcellular localization Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.70 38000
-
 SDS-PAGE Bos taurus
1.3.1.70 46750
-
 cloned cDNA, calculated from amino-acid sequence. The discrepancy between the apparent molecular mass of 38000 Da estimated by SDS-PAGE and the calculated molecular mass may be due to an aberrant electrophoretic migration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.70 additional information Homo sapiens 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway ?
-
 ?
1.3.1.70 additional information Bos taurus 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway ?
-
 ?
1.3.1.70 additional information Homo sapiens important essential enzyme of cholesterol biosynthesis from lanosterol ?
-
 ?
1.3.1.70 additional information Bos taurus important essential enzyme of cholesterol biosynthesis from lanosterol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.70 Bos taurus
-
 bovine
-
1.3.1.70 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.70
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.70 brain
-
 Bos taurus
-
1.3.1.70 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.70 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH
-
 Homo sapiens 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+
-
 ?
1.3.1.70 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH C29DELTA8,14,24 Bos taurus 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+
-
 ?
1.3.1.70 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + NADPH
-
 Homo sapiens 4,4-dimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+
-
 ?
1.3.1.70 4,4-dimethyl-5alpha-cholesta-8,14-dien-3beta-ol + NADPH
-
 Bos taurus 4,4-dimethyl-5alpha-cholesta-8-en-3beta-ol + NADP+
-
 ?
1.3.1.70 5alpha-cholesta-8,14-dien-3beta-ol + NADPH DELTA8,14-cholestadien-3beta-ol Bos taurus 5alpha-cholesta-8-en-3beta-ol + NADP+
-
 ?
1.3.1.70 5alpha-cholesta-8,14-dien-3beta-ol + NADPH C27DELTA8,14 Bos taurus 5alpha-cholesta-8-en-3beta-ol + NADP+
-
 ?
1.3.1.70 additional information enzyme acts on a range of steroids with a 14(15)-double bond Homo sapiens ?
-
 ?
1.3.1.70 additional information enzyme acts on a range of steroids with a 14(15)-double bond Bos taurus ?
-
 ?
1.3.1.70 additional information 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway Homo sapiens ?
-
 ?
1.3.1.70 additional information 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway Bos taurus ?
-
 ?
1.3.1.70 additional information important essential enzyme of cholesterol biosynthesis from lanosterol Homo sapiens ?
-
 ?
1.3.1.70 additional information important essential enzyme of cholesterol biosynthesis from lanosterol Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.70 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.70 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.70 NADPH
-
 Homo sapiens
1.3.1.70 NADPH
-
 Bos taurus