BRENDA - Enzyme Database

Aldehyde dehydrogenase from acetic acid bacteria, membrane-bound

Ameyama, M.; Adachi, O.; Methods Enzymol. 89, 491-497 (1982)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.5.2
2.9
-
acetaldehyde
-
Acetobacter aceti
1.2.5.2
3.3
-
acetaldehyde
-
Gluconobacter oxydans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.5.2
137000
-
3 components, MW 78000 dehydrogenase protein, MW 45000 cytochrome component, MW 14000 component with unknown function, SDS-PAGE
Acetobacter aceti
1.2.5.2
140000
-
2 components, MW 86000 dehydrogenase protein, MW 55000 cytochrome c, SDS-PAGE
Gluconobacter oxydans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.5.2
acetaldehyde + a quinone + H2O
Gluconobacter oxydans
-
acetate + a quinol
-
Gluconobacter oxydans
?
1.2.5.2
acetaldehyde + a quinone + H2O
Acetobacter aceti
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
acetate + a quinol
-
Acetobacter aceti
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.5.2
Acetobacter aceti
-
-
-
1.2.5.2
Gluconobacter oxydans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.5.2
solubilization with Triton X-100, DEAE-cellulose, CM-cellulose
Gluconobacter oxydans
1.2.5.2
solubilization with Triton X-100/cetylpyridinium chloride, PEG precipitation, DEAE-cellulose, hydroxyapatite
Acetobacter aceti
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.5.2
430
-
-
Gluconobacter oxydans
1.2.5.2
654
-
-
Acetobacter aceti
Storage Stability
EC Number
Storage Stability
Organism
1.2.5.2
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Acetobacter aceti
1.2.5.2
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Gluconobacter oxydans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.5.2
acetaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
acetate + a quinol
-
288471
Gluconobacter oxydans
?
1.2.5.2
acetaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
acetate + a quinol
-
288471
Acetobacter aceti
?
1.2.5.2
benzaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
benzoate + a quinol
-
288471
Gluconobacter oxydans
?
1.2.5.2
benzaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors, 5% activity of acetaldehyde oxidation
288471
Acetobacter aceti
benzoate + a quinol
-
288471
Acetobacter aceti
?
1.2.5.2
butanal + a quinone + H2O
-
288471
Gluconobacter oxydans
butanoate + a quinone
-
288471
Gluconobacter oxydans
?
1.2.5.2
butanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
butanoate + a quinone
-
288471
Acetobacter aceti
?
1.2.5.2
hexanal + a quinone + H2O
-
288471
Gluconobacter oxydans
hexanoate + a quinol
-
288471
Gluconobacter oxydans
?
1.2.5.2
hexanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
hexanoate + a quinol
-
288471
Acetobacter aceti
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.5.2
4
-
-
Gluconobacter oxydans
1.2.5.2
5
-
-
Acetobacter aceti
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.5.2
pyrroloquinoline quinone
-
Acetobacter aceti
1.2.5.2
pyrroloquinoline quinone
-
Gluconobacter oxydans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.5.2
pyrroloquinoline quinone
-
Acetobacter aceti
1.2.5.2
pyrroloquinoline quinone
-
Gluconobacter oxydans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.5.2
2.9
-
acetaldehyde
-
Acetobacter aceti
1.2.5.2
3.3
-
acetaldehyde
-
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.5.2
137000
-
3 components, MW 78000 dehydrogenase protein, MW 45000 cytochrome component, MW 14000 component with unknown function, SDS-PAGE
Acetobacter aceti
1.2.5.2
140000
-
2 components, MW 86000 dehydrogenase protein, MW 55000 cytochrome c, SDS-PAGE
Gluconobacter oxydans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.5.2
acetaldehyde + a quinone + H2O
Gluconobacter oxydans
-
acetate + a quinol
-
Gluconobacter oxydans
?
1.2.5.2
acetaldehyde + a quinone + H2O
Acetobacter aceti
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
acetate + a quinol
-
Acetobacter aceti
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.5.2
solubilization with Triton X-100, DEAE-cellulose, CM-cellulose
Gluconobacter oxydans
1.2.5.2
solubilization with Triton X-100/cetylpyridinium chloride, PEG precipitation, DEAE-cellulose, hydroxyapatite
Acetobacter aceti
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.5.2
430
-
-
Gluconobacter oxydans
1.2.5.2
654
-
-
Acetobacter aceti
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.5.2
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Acetobacter aceti
1.2.5.2
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.5.2
acetaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
acetate + a quinol
-
288471
Gluconobacter oxydans
?
1.2.5.2
acetaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
acetate + a quinol
-
288471
Acetobacter aceti
?
1.2.5.2
benzaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
benzoate + a quinol
-
288471
Gluconobacter oxydans
?
1.2.5.2
benzaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors, 5% activity of acetaldehyde oxidation
288471
Acetobacter aceti
benzoate + a quinol
-
288471
Acetobacter aceti
?
1.2.5.2
butanal + a quinone + H2O
-
288471
Gluconobacter oxydans
butanoate + a quinone
-
288471
Gluconobacter oxydans
?
1.2.5.2
butanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
butanoate + a quinone
-
288471
Acetobacter aceti
?
1.2.5.2
hexanal + a quinone + H2O
-
288471
Gluconobacter oxydans
hexanoate + a quinol
-
288471
Gluconobacter oxydans
?
1.2.5.2
hexanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
hexanoate + a quinol
-
288471
Acetobacter aceti
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.5.2
4
-
-
Gluconobacter oxydans
1.2.5.2
5
-
-
Acetobacter aceti