Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kunow, J.; Linder, D.; Thauer, R.K.
    Pyruvate:ferredoxin oxidoreductase from the sulfate-reducing Archaeoglobus fulgidus: molecular composition, catalytic properties, and sequence alignments (1995), Arch. Microbiol., 163, 21-28.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.1 dioxygen the enzyme is inactivated by dioxygen. Less than 50% of the original activity remains when the protein (0.23 mg/ml in 50 mM Tris/HCl pH 7.5) is exposed to air for 80 min at 25°C. Attempts to reactivate the enzyme by anoxic incubation in the presence of 2 mM dithiothreitol fail Archaeoglobus fulgidus
1.2.7.1 KCl 0.5 M, 50% inhibition Archaeoglobus fulgidus
1.2.7.1 additional information the enzyme is not inhibited by sodium nitrite (50 mM) or carbon monoxide (100% CO as gas phase) Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.01
-
 oxidized ferredoxin pH 7.5, 65°C Archaeoglobus fulgidus
1.2.7.1 0.02
-
 CoA pH 7.5, 65°C Archaeoglobus fulgidus
1.2.7.1 0.3
-
 pyruvate pH 7.5, 65°C Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.1 Iron per mol, the enzyme contains 9 mol of non-heme iron, and 8 mol of acid-labile sulfur. No inhibition by sodium arsenite Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 13000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
1.2.7.1 25000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
1.2.7.1 33000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
1.2.7.1 45000
-
 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus
1.2.7.1 120000
-
 gel filtration Archaeoglobus fulgidus
1.2.7.1 125000
-
 polyacrylamide gradient gel electrophoresis Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Archaeoglobus fulgidus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 the enzyme is inactivated by O2. Less than 50% of the original activity remains when the protein (0.23 mg/ml in 50 mM Tris/HCl pH 7.5) is exposed to air for 80 min at 25°C. Attempts to reactivate the enzyme by anoxic incubation in the presence of 2 mM dithiothreitol fail Archaeoglobus fulgidus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin
-
 Archaeoglobus fulgidus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen the enzyme does not catalyze the oxidation of 2-oxoglutarate, indolepyruvate, phenylpyruvate, glyoxylate, and hydroxypyruvate Archaeoglobus fulgidus acetyl-CoA + CO2 + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 tetramer 1 * 45000 + 1 * 33000 + 1 * 25000 + 1 * 13000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 pyruvate:ferredoxin oxidoreductase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 65
-
 assay at Archaeoglobus fulgidus
1.2.7.1 90
-
 above Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.1 90
-
 60 min, in the presence of 2 M KCl, 50% loss of activity. In absence of salt 50% loss of activity after 50 min Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 7.5
-
 assay at Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.1 thiamine diphosphate per mol, the enzyme contains 0.8 mol thiamine diphosphate Archaeoglobus fulgidus