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Literature summary extracted from

  • Blamey, J.M.; Adams, M.W.W.
    Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus (1993), Biochim. Biophys. Acta, 1161, 19-27.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.7.1 purified enzyme, stored for one year under liquid N2, no loss of activity if thawed anaerobically Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.1 carbon monoxide 0.0072 mM, 50% inhibition Pyrococcus furiosus
1.2.7.1 nitrite
-
 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.094
-
 oxidized ferredoxin pH 8.4, 80°C Pyrococcus furiosus
1.2.7.1 0.11
-
 CoA pH 8.4, 80°C Pyrococcus furiosus
1.2.7.1 0.46
-
 pyruvate pH 8.4, 80°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.1 Iron 8.3 gatoms/100000 g of protein Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 24000
-
 1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.2.7.1 31000
-
 1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.2.7.1 45000
-
 1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.2.7.1 100000
-
 electrophoresis on non-denaturing gels Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin Pyrococcus furiosus
-
 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 20
-
 pH 8.4, 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 additional information no activity with 2-oxoglutarate, phenyl pyruvate or indole pyruvate Pyrococcus furiosus ?
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin
-
 Pyrococcus furiosus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen
-
 Pyrococcus furiosus acetyl-CoA + CO2 + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 trimer 1 * 45000 + 1 * 31000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 pyruvate ferredoxin oxidoreductase
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.1 80
-
 50% loss of activity after 23 min Pyrococcus furiosus
1.2.7.1 90
-
 50% loss of activity after 18 min Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.1 thiamine diphosphate 0.5-0.7 per mol of enzyme Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.7.1 11
-
 nitrite pH 8.4, 80°C Pyrococcus furiosus