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Literature summary extracted from

  • Zhang, Q.; Iwaaki, T.; Wakagi, T.; Oshima, T.
    2-Oxoacid:ferredoxin oxidoreductase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7 (1996), J. Biochem., 120, 587-599.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.11
-
 Sulfolobus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.11 0.25
-
 pyruvate pH 6.8, 50°C Sulfolobus sp.
1.2.7.11 0.87
-
 2-oxoglutarate pH 6.8, 50°C Sulfolobus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.11 Iron contains one [4Fe-4S]2+,1+ cluster per alpha/beta structure Sulfolobus sp.
1.2.7.11 Mg2+ contains 1.4 mol of Mg per mol of protein Sulfolobus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.11 Sulfolobus sp. P72578 and P72579 P72578: subunit alpha, P72579: subunit beta
-
1.2.7.11 Sulfolobus sp. 7 P72578 and P72579 P72578: subunit alpha, P72579: subunit beta
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.11 purified enzyme is stable against oxygen Sulfolobus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.11
-
 Sulfolobus sp.

Storage Stability

EC Number Storage Stability Organism
1.2.7.11 -80°C, purified enzyme can be stored after dialysis against 20 mM potassium phosphate buffer, pH 6.8 Sulfolobus sp.
1.2.7.11 20°C, 4°C or -80°C, activity in cytosolic fraction is not very stable Sulfolobus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.11 2-oxobutyrate + CoA + oxidized ferredoxin
-
 Sulfolobus sp. propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.11 2-oxobutyrate + CoA + oxidized ferredoxin
-
 Sulfolobus sp. 7 propanoyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.11 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
 Sulfolobus sp. succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.11 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
 Sulfolobus sp. 7 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.11 pyruvate + CoA + 2 oxidized ferredoxin Vmax/Km is 1.9fold higher than that for 2-oxoglutarate Sulfolobus sp. acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.11 pyruvate + CoA + 2 oxidized ferredoxin Vmax/Km is 1.9fold higher than that for 2-oxoglutarate Sulfolobus sp. 7 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.11 50
-
 assay at Sulfolobus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.11 6.8
-
 assay at Sulfolobus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.11 thiamine diphosphate the enzyme contains one thiamine diphosphate per alphabeta structure Sulfolobus sp.