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Literature summary extracted from

  • Pundir, C.S.; Verma, U.
    Isolation, purification, immobilization of oxalate oxidase and its clinical applications (1993), Hindustan Antibiot. Bull., 35, 173-182.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.3.4 medicine colorimetric or UV-determination of oxalic acid in biological fluids and beverages Hordeum vulgare

General Stability

EC Number General Stability Organism
1.2.3.4 immobilized on zirkonia coated alkylamine glass, retains 97% of enzyme activity Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 Cl- 1 M concentration 75% inhibition Musa acuminata
1.2.3.4 Fe3+ 10 mM concentration 35% inhibition Musa acuminata
1.2.3.4 NaCl at 1 mM concentration 43% activity retained by free protein, 85% activity retained by immobilized protein Hordeum vulgare
1.2.3.4 Pb2+ 2 mM concentration 57% inhibition, 20 mM concentration 89% inhibition Musa acuminata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 0.37
-
 oxalate immobilized protein Hordeum vulgare
1.2.3.4 0.42
-
 oxalate
-
 Hordeum vulgare
1.2.3.4 1.88
-
 oxalate
-
 Musa acuminata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.3.4 cytosol
-
 Hordeum vulgare 5829
-
1.2.3.4 cytosol
-
 Musa acuminata 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Hordeum vulgare
-
-
-
1.2.3.4 Musa acuminata
-
 banana
-

Storage Stability

EC Number Storage Stability Organism
1.2.3.4 4°C, under 3 months no loss of activity by free protein, over 2 years immobilized protein Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Hordeum vulgare 2 CO2 + H2O2
-
 ir
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Musa acuminata 2 CO2 + H2O2
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.4 60
-
-
 Hordeum vulgare
1.2.3.4 60
-
 70% activity retained Musa acuminata
1.2.3.4 75
-
 80% wild type and 20% immobilized protein Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 3.2
-
-
 Hordeum vulgare
1.2.3.4 4
-
 immobilized protein Hordeum vulgare