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Literature summary extracted from

  • Blake, R.; Hager, L.P.
    Activation of pyruvate oxidase by monomeric and micellar amphiphiles (1978), J. Biol. Chem., 253, 1963-1971.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.5.1 additional information the enzyme activity is stimulated 20- to 50fold, if the enzyme is removed from the membrane particulate fraction of the cell by incubation with a wide variety of amphiphiles Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.5.1 membrane peripheral membrane enzyme Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.5.1 anionic detergents the enzyme activity is stimulated 20- to 50fold, if the enzyme is removed from the membrane particulate fraction of the cell by incubation with a wide variety of amphiphiles Escherichia coli
1.2.5.1 Cationic detergents the enzyme activity is stimulated 20- to 50fold, if the enzyme is removed from the membrane particulate fraction of the cell by incubation with a wide variety of amphiphiles Escherichia coli
1.2.5.1 Zwitterionic detergents the enzyme activity is stimulated 20- to 50fold, if the enzyme is removed from the membrane particulate fraction of the cell by incubation with a wide variety of amphiphiles Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.5.1 pyruvate + ferricytochrome b1 + H2O Escherichia coli the natural electron acceptor for the reduced enzyme is a cell-membrane-associated electron transport system including both ubiquinone-6- and cytochrome b1, with oxygen being the terminal electron acceptor acetate + CO2 + ferrocytochrome b1
-
 ?
1.2.5.1 pyruvate + ubiquinol-6 + H2O Escherichia coli the natural electron acceptor for the reduced enzyme is a cell-membrane-associated electron transport system including both ubiquinone-6 and cytochrome b1, with oxygen being the terminal electron acceptor acetate + CO2 + ubiquinol-6
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.5.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.1 purified to homogeneity in the absence of detergents Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.5.1 additional information
-
 the enzyme activity is stimulated 20- to 50fold, if the enzyme is removed from the membrane particulate fraction of the cell by incubation with a wide variety of amphiphiles Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.5.1 pyruvate + ferricyanide + H2O
-
 Escherichia coli acetate + CO2 + ferrocyanide
-
 ?
1.2.5.1 pyruvate + ferricytochrome b1 + H2O the natural electron acceptor for the reduced enzyme is a cell-membrane-associated electron transport system including both ubiquinone-6- and cytochrome b1, with oxygen being the terminal electron acceptor Escherichia coli acetate + CO2 + ferrocytochrome b1
-
 ?
1.2.5.1 pyruvate + ubiquinol-6 + H2O the natural electron acceptor for the reduced enzyme is a cell-membrane-associated electron transport system including both ubiquinone-6 and cytochrome b1, with oxygen being the terminal electron acceptor Escherichia coli acetate + CO2 + ubiquinol-6
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.5.1 pyruvate oxidase
-
 Escherichia coli