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Literature summary extracted from
- Glyceraldehyde-3-phosphate dehydrogenase from the latex of Hevea brasiliensis. Comparative study with its phosphorylating homologue (1972), Eur. J. Biochem., 31, 255-265.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.9 | ADP | 3 mM, in coupled assay with aldolase | Hevea brasiliensis |  |
| 1.2.1.9 | AMP | 3 mM, in coupled assay with aldolase | Hevea brasiliensis | |
| 1.2.1.9 | ATP | 3 mM, in coupled assay with aldolase | Hevea brasiliensis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.9 | Ca2+ | - |
Hevea brasiliensis | |
| 1.2.1.9 | iodoacetamide | - |
Hevea brasiliensis | |
| 1.2.1.9 | Mg2+ | - |
Hevea brasiliensis | |
| 1.2.1.9 | NADH | - |
Hevea brasiliensis | |
| 1.2.1.9 | phosphate | - |
Hevea brasiliensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.9 | 0.01 | - |
NADP+ | coupled assay with aldolase | Hevea brasiliensis | |
| 1.2.1.9 | 1.2 | - |
NAD+ | glyceraldehyde 3-phosphate | Hevea brasiliensis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.2.1.9 | additional information | aqueous phase of latex after centrifugation at 38000 x g | Hevea brasiliensis | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.9 | 190000 | 200000 | gel filtration | Hevea brasiliensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.9 | D-glyceraldehyde-3-phosphate + NADP+ | Hevea brasiliensis | - |
D-3-phosphoglycerate + NADPH | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.9 | Hevea brasiliensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.9 | - |
Hevea brasiliensis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.2.1.9 | latex | - |
Hevea brasiliensis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.9 | 32.4 | - |
purified enzyme | Hevea brasiliensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.9 | D-glyceraldehyde 3-phosphate + NAD+ + H2O | - |
Hevea brasiliensis | 3-phospho-D-glycerate + NADH + H+ | - |
r | |
| 1.2.1.9 | D-glyceraldehyde-3-phosphate + NADP+ | - |
Hevea brasiliensis | D-3-phosphoglycerate + NADPH | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.1.9 | tetramer | - |
Hevea brasiliensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.9 | 60 | - |
6 min, 89% inactivation | Hevea brasiliensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.9 | 8.6 | - |
- |
Hevea brasiliensis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.9 | NAD+ | low activity | Hevea brasiliensis | |
| 1.2.1.9 | NADP+ | - |
Hevea brasiliensis | |
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Release 2023.1 (January 2023)
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