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Literature summary extracted from

  • Stachow, C.S.; Stevenson, I.L.; Day, D.
    Purification and properties of nicotinamide adenine dinucleotide phosphate-specific benzaldehyde dehydrogenase from Pseudomonas (1967), J. Biol. Chem., 242, 5294-5300.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.7 NH4+ or K+ or Rb+ required for maximal activity Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.7 N-ethylmaleimide
-
 Pseudomonas fluorescens
1.2.1.7 p-hydroxymercuribenzoate
-
 Pseudomonas fluorescens
1.2.1.7 Zn2+
-
 Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.7 0.0002
-
 benzaldehyde
-
 Pseudomonas fluorescens
1.2.1.7 0.037
-
 NADP+
-
 Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.7 K+ or NH4+ or Rb+ required for maximal activity Pseudomonas fluorescens
1.2.1.7 Rb+ or K+ or NH4+ required for maximal activity Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.7 200000
-
 sucrose density gradient centrifugation Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.7 4-hydroxybenzaldehyde + NADP+ + H2O Pseudomonas fluorescens
-
 4-hydroxybenzoate + NADPH + H+
-
 ?
1.2.1.7 benzaldehyde + NADP+ + H2O Pseudomonas fluorescens
-
 benzoate + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.7 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.7
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.7 2.14
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.2.1.7 -17°C, pH 7.2, 10 mM dithiothreitol, several months Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.7 4-hydroxybenzaldehyde + NADP+ + H2O
-
 Pseudomonas fluorescens 4-hydroxybenzoate + NADPH + H+
-
 ?
1.2.1.7 benzaldehyde + NADP+ + H2O
-
 Pseudomonas fluorescens benzoate + NADPH
-
 ?
1.2.1.7 isovanillin + NADP+ + H2O
-
 Pseudomonas fluorescens 3-hydroxy-4-methoxybenzoate + NADPH
-
 ?
1.2.1.7 salicylaldehyde + NADP+ + H2O
-
 Pseudomonas fluorescens salicylic acid + NADPH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.7 additional information
-
 heat-labile Pseudomonas fluorescens
1.2.1.7 50
-
 inactivation after 5 min Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.7 8.5
-
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.7 NADP+
-
 Pseudomonas fluorescens