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Literature summary extracted from

  • Van Ophem, P.W.; Van Beeumen, J.; Duine, J.A.
    NAD-linked, factor-dependent formaldehyde dehydrogenase or trimeric, zinc-containing, long-chain alcohol dehydrogenase from Amycolatopsis methanolica (1992), Eur. J. Biochem., 206, 511-518.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.306 factor unidentified factor that is required for maximal activity with formaldehyde as substrate, only 4% activity in its absence, oxidation of alcohols do not require the factor Amycolatopsis methanolica
1.1.1.306 methanol can replace an unidentified activating factor Amycolatopsis methanolica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.192 1,10-phenanthroline
-
 Amycolatopsis methanolica
1.1.1.192 CuCl2
-
 Amycolatopsis methanolica
1.1.1.192 HgCl2
-
 Amycolatopsis methanolica
1.1.1.192 KCN
-
 Amycolatopsis methanolica
1.1.1.192 additional information inhibition differs for ethanol and formaldehyde as substrates Amycolatopsis methanolica
1.1.1.306 1,10-phenanthroline 80% inhibition at 5 mM Amycolatopsis methanolica
1.1.1.306 2-(cyclohexylamino)ethanesulfonic acid-NaOH 25 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 Amycolatopsis methanolica
1.1.1.306 acetaldehyde 15% inhibition at 1 mM Amycolatopsis methanolica
1.1.1.306 Cu2+ complete inhibition at 1 mM Amycolatopsis methanolica
1.1.1.306 glycine-NaOH 100 mM at pH 9.0: 40% of the activity compared to 0.1 M diphosphate, pH 9.0 Amycolatopsis methanolica
1.1.1.306 Hg2+ complete inhibition at 0.1 mM Amycolatopsis methanolica
1.1.1.306 KCN 55% inhibition at 2 mM Amycolatopsis methanolica
1.1.1.306 Na2B4O7-HCl 100 mM at pH 9.0: no activity Amycolatopsis methanolica
1.1.1.306 Tris-HCl 50 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 Amycolatopsis methanolica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.192 1.2
-
 1-Octanol
-
 Amycolatopsis methanolica
1.1.1.192 1.7
-
 12-Hydroxydodecanoic acid
-
 Amycolatopsis methanolica
1.1.1.192 3.4
-
 1-Hexanol
-
 Amycolatopsis methanolica
1.1.1.192 26.5
-
 1-Pentanol
-
 Amycolatopsis methanolica
1.1.1.192 84
-
 1-butanol
-
 Amycolatopsis methanolica
1.1.1.192 324
-
 1-propanol
-
 Amycolatopsis methanolica
1.1.1.192 343
-
 ethanol
-
 Amycolatopsis methanolica
1.1.1.306 1.2
-
 1-Octanol
-
 Amycolatopsis methanolica
1.1.1.306 1.7
-
 12-Hydroxydodecanoic acid
-
 Amycolatopsis methanolica
1.1.1.306 3.4
-
 1-Hexanol
-
 Amycolatopsis methanolica
1.1.1.306 9.6
-
 formaldehyde
-
 Amycolatopsis methanolica
1.1.1.306 26.5
-
 1-Pentanol
-
 Amycolatopsis methanolica
1.1.1.306 84
-
 1-butanol
-
 Amycolatopsis methanolica
1.1.1.306 324
-
 1-propanol
-
 Amycolatopsis methanolica
1.1.1.306 343
-
 ethanol
-
 Amycolatopsis methanolica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.192 Zinc 6.1 atoms/enzyme molecule Amycolatopsis methanolica
1.1.1.306 Zn2+ 6 atoms Zn/enzyme molecule Amycolatopsis methanolica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.192 40000
-
 3 * 40000, SDS-PAGE Amycolatopsis methanolica
1.1.1.192 117000 120000 PAGE, gel filtration Amycolatopsis methanolica
1.1.1.306 40000
-
 3 * 40000, SDS-PAGE Amycolatopsis methanolica
1.1.1.306 120000
-
 gel filtration Amycolatopsis methanolica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.192 long-chain alcohol + NAD+ + H2O Amycolatopsis methanolica enzyme also uses formaldehyde and short chain aliphatic alcohols long-chain carboxylate + NADH
-
 ?
1.1.1.306 formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+ Amycolatopsis methanolica
-
 S-formylmycothiol + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.192 Amycolatopsis methanolica
-
-
-
1.1.1.306 Amycolatopsis methanolica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.306 to homogeneity Amycolatopsis methanolica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.306 cell culture induction of enzyme and factor synthesis only if cells grown in methanol-containing medium Amycolatopsis methanolica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.306 0.03
-
 cell free extract, in the absence of an unknown activating factor Amycolatopsis methanolica
1.1.1.306 0.08
-
 cell free extract, in the presence of an unknown activating factor Amycolatopsis methanolica
1.1.1.306 6.9
-
 substrate: ethanol Amycolatopsis methanolica
1.1.1.306 15
-
 substrate: formaldehyde Amycolatopsis methanolica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.192 1-octanol + 2 NAD+ + H2O
-
 Amycolatopsis methanolica octanoic acid + 2 NADH + 2 H+
-
 r
1.1.1.192 12-hydroxydodecanoic acid + NAD+ + H2O
-
 Amycolatopsis methanolica ? + NADH
-
 ?
1.1.1.192 ethanol + NAD+ + H2O
-
 Amycolatopsis methanolica ethanal + NADH
-
 ?
1.1.1.192 long-chain alcohol + NAD+ + H2O enzyme also uses formaldehyde and short chain aliphatic alcohols Amycolatopsis methanolica long-chain carboxylate + NADH
-
 ?
1.1.1.192 n-butanol + NAD+ + H2O
-
 Amycolatopsis methanolica n-butanal + NADH
-
 ?
1.1.1.192 n-hexanol + NAD+ + H2O
-
 Amycolatopsis methanolica n-hexanal + NADH
-
 ?
1.1.1.192 n-pentanol + NAD+ + H2O
-
 Amycolatopsis methanolica n-pentanal + NADH
-
 ?
1.1.1.192 n-propanol + NAD+ + H2O
-
 Amycolatopsis methanolica n-propanal + NADH
-
 ?
1.1.1.306 1-butanol + NAD+
-
 Amycolatopsis methanolica n-butanal + NADH
-
 ?
1.1.1.306 1-hexanol + NAD+
-
 Amycolatopsis methanolica hexanal + NADH + H+
-
 r
1.1.1.306 1-octanol + NAD+
-
 Amycolatopsis methanolica octanal + NADH + H+
-
 r
1.1.1.306 1-pentanol + NAD+
-
 Amycolatopsis methanolica pentanal + NADH + H+
-
 r
1.1.1.306 1-propanol + NAD+
-
 Amycolatopsis methanolica propanal + NADH + H+
-
 r
1.1.1.306 12-hydroxydodecanoic acid + NADH
-
 Amycolatopsis methanolica ? + NAD+
-
 ?
1.1.1.306 ethanol + NAD+
-
 Amycolatopsis methanolica ethanal + NADH + H+
-
 r
1.1.1.306 formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+
-
 Amycolatopsis methanolica S-formylmycothiol + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.192 More N-terminal sequence Amycolatopsis methanolica
1.1.1.192 trimer 3 * 40000, SDS-PAGE Amycolatopsis methanolica
1.1.1.306 trimer 3 * 40000, SDS-PAGE Amycolatopsis methanolica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.306 FD-FA1DH
-
 Amycolatopsis methanolica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.306 9
-
 formaldehyde as substrate Amycolatopsis methanolica
1.1.1.306 10.2
-
 alcohols as substrates Amycolatopsis methanolica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.306 NAD+
-
 Amycolatopsis methanolica