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Literature summary extracted from

  • Barbour, M.G.; Bayly, R.C.
    Control of meta-cleavage degradation of 4-hydroxyphenylacetate in Pseudomonas putida (1981), J. Bacteriol., 147, 844-850.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.9 additional information several mutants are created, e.g. strains with enzyme defectives for analysis of the 4-hydroxyphenylacetate pathway Pseudomonas putida

General Stability

EC Number General Stability Organism
1.2.1.60 heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Pseudomonas putida involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Pseudomonas putida NCIB 9865 involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Pseudomonas putida induced by 3,4-dihydroxyphenylacetate 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 Pseudomonas putida degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 Pseudomonas putida NCIB 9865 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.60 Pseudomonas putida
-
-
-
1.2.1.60 Pseudomonas putida NCIB 9865
-
-
-
1.13.11.15 Pseudomonas putida
-
 mutant strains P23XC1, P23XC5 and P23XC7, no activity in mutant strain P23X2
-
1.13.11.15 Pseudomonas putida
-
 wild-type strain P23X1
-
1.14.14.9 Pseudomonas putida
-
 P23X1, NCIB 9865
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.60 cell culture strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.60 0.07
-
-
 Pseudomonas putida
1.14.14.9 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Pseudomonas putida 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Pseudomonas putida NCIB 9865 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Pseudomonas putida 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 induced by 3,4-dihydroxyphenylacetate Pseudomonas putida 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Pseudomonas putida NCIB 9865 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Pseudomonas putida NCIB 9865 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway Pseudomonas putida NCIB 9865 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 additional information strict substrate specificity Pseudomonas putida ?
-
 ?
1.14.14.9 additional information strict substrate specificity Pseudomonas putida NCIB 9865 ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.60 NAD+
-
 Pseudomonas putida