EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.60 | NADH | competitive inhibition with respect to NAD+, Ki: 0.027 mM | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.60 | 0.009 | - |
5-carboxymethyl-2-hydroxymuconate semialdehyde | - |
Pseudomonas putida | |
1.2.1.60 | 0.02 | - |
NAD+ | - |
Pseudomonas putida | |
1.2.1.60 | 0.048 | - |
NAD+ | - |
Pseudomonas putida | |
1.2.1.60 | 1.2 | - |
NADP+ | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.60 | 52500 | - |
3 * 52500, SDS-PAGE | Pseudomonas putida |
1.2.1.60 | 200000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Pseudomonas putida | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.60 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.60 | to homogeneity, chromatography techniques | Pseudomonas putida |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.60 | cell culture | - |
Pseudomonas putida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.60 | 11 | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.60 | frozen, 12 months | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Pseudomonas putida | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ | 18% of the activity compared to reduction with NAD+ | Pseudomonas putida | 5-carboxymethyl-2-hydroxymuconate + NADPH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.60 | tetramer | 3 * 52500, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.60 | 37 | - |
stable for 30 min | Pseudomonas putida |
1.2.1.60 | 47 | - |
complete loss of activity after 20 min | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.60 | 40 | - |
5-carboxymethyl-2-hydroxymuconate semialdehyde | - |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.60 | 7.8 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.60 | NAD+ | strict dependency for NAD+ reduction | Pseudomonas putida | |
1.2.1.60 | NADP+ | 82% lower activity compared to NAD+ | Pseudomonas putida |